microRNA-664 enhances proliferation, migration and invasion of lung cancer cells
| Autor: | Guoping Chen, Chuanchuan Li, Yue Shu, Feng Yuan, Yanhui Xu, Jing Luo, Xinhai Zhu, Sheng Ju, Lilong Xia |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
A549 cell Cancer Research Oncogene Cancer General Medicine Articles Biology respiratory system medicine.disease medicine.disease_cause Metastasis respiratory tract diseases 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Immunology and Microbiology (miscellaneous) 030220 oncology & carcinogenesis microRNA medicine Cancer research Lung cancer Carcinogenesis Protein kinase B |
| Zdroj: | Experimental and therapeutic medicine. 13(6) |
| ISSN: | 1792-0981 |
| Popis: | Altered microRNA (miR) expression serves an important role in the development and progression of lung cancer. In the present study, the effect of miR-664 on proliferation, migration and invasion of lung cancer cells was assessed. The proliferation of lung cancer cells with an overexpression of miR-664 was examined via MTT assay. The Caspase-Glo3/7 assay was used to examine the effect of miR-664 on cisplatin-induced apoptosis in lung cancer cells. The migration and invasion of lung cancer cells were assessed by Transwell migration and matrigel invasion assays. Western blot analysis was used to examine the protein expression levels. miR-664 improved the proliferation of lung cancer cells and inhibited cisplatin-induced apoptosis of A549 and A427 cells. Furthermore, altered expression of miR-664 affected migration and invasion of lung cancer cells. In addition, a miR-664 mimic decreased E-cadherin expression and increased vementin and Snail expression in lung cancer cells. Notably, the expression level of protein kinase B in A549 cells was changed following altered expression of miR-664. The results of the present study suggest that miR-664 serves an essential role in tumor development and progression in lung cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|