Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state

Autor: Fabiana Passaro, Luca Cozzuto, Silvia Parisi, Dario Antonini, Lucio Pastore, Luigi Aloia, Vincenzo De Simone, Tommaso Russo, Carolina Tarantino, Simona Ciriello
Přispěvatelé: Parisi, Silvia, Cozzuto, Luca, Tarantino, Carolina, Passaro, Fabiana, Ciriello, S, Aloia, Luigi, Antonini, Dario, DE SIMONE, Vincenzo, Pastore, Lucio, Russo, Tommaso
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Pluripotent Stem Cells
Chromatin Immunoprecipitation
Physiology
Cellular differentiation
Blotting
Western
Kruppel-Like Transcription Factors
Plant Science
Biology
Regulon
General Biochemistry
Genetics and Molecular Biology
Kruppel-Like Factor 4
Mice
Structural Biology
Animals
Transcription factor
lcsh:QH301-705.5
Embryonic Stem Cells
Ecology
Evolution
Behavior and Systematics
reproductive and urinary physiology
Regulation of gene expression
Agricultural and Biological Sciences(all)
Biochemistry
Genetics and Molecular Biology(all)
Microarray analysis techniques
Gene Expression Regulation
Developmental
RNA-Binding Proteins
Cell Differentiation
Cell Biology
Klf5
pluripotency
Microarray Analysis
embryonic stem cell
Embryonic stem cell
Cell biology
Phenotype
Gene Expression Regulation
lcsh:Biology (General)
KLF4
KLF2
embryonic structures
Commentary
biological phenomena
cell phenomena
and immunity
General Agricultural and Biological Sciences
Chromatin immunoprecipitation
Developmental Biology
Biotechnology
Research Article
Zdroj: BMC Biology, Vol 8, Iss 1, p 128 (2010)
BMC Biology
ISSN: 1741-7007
Popis: Background A growing body of evidence has shown that Krüppel-like transcription factors play a crucial role in maintaining embryonic stem cell (ESC) pluripotency and in governing ESC fate decisions. Krüppel-like factor 5 (Klf5) appears to play a critical role in these processes, but detailed knowledge of the molecular mechanisms of this function is still not completely addressed. Results By combining genome-wide chromatin immunoprecipitation and microarray analysis, we have identified 161 putative primary targets of Klf5 in ESCs. We address three main points: (1) the relevance of the pathways governed by Klf5, demonstrating that suppression or constitutive expression of single Klf5 targets robustly affect the ESC undifferentiated phenotype; (2) the specificity of Klf5 compared to factors belonging to the same family, demonstrating that many Klf5 targets are not regulated by Klf2 and Klf4; and (3) the specificity of Klf5 function in ESCs, demonstrated by the significant differences between Klf5 targets in ESCs compared to adult cells, such as keratinocytes. Conclusions Taken together, these results, through the definition of a detailed list of Klf5 transcriptional targets in mouse ESCs, support the important and specific functional role of Klf5 in the maintenance of the undifferentiated ESC phenotype. See: http://www.biomedcental.com/1741-7007/8/125
Databáze: OpenAIRE
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