Detection of leptospiral antigen in the human liver and kidney using an immunoperoxidase staining procedure
| Autor: | T. De Brito, M. Regina Vianna, P. H. Yasuda, V. A. Ferreira Alves |
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| Rok vydání: | 1987 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Kidney Pathology and Forensic Medicine Immunoenzyme Techniques Antigen Leptospira Biopsy medicine Humans Antigens Bacterial biology medicine.diagnostic_test Immunoperoxidase Mononuclear phagocyte system Middle Aged medicine.disease biology.organism_classification Leptospirosis Staining medicine.anatomical_structure Liver Leptospira interrogans Weil Disease |
| Zdroj: | The Journal of Pathology. 151:125-131 |
| ISSN: | 1096-9896 0022-3417 |
| DOI: | 10.1002/path.1711510205 |
| Popis: | Detection of leptospiral antigen using an immunoperoxidase staining (IP) procedure was carried out on fifteen samples of human liver, (nine from autopsies and six from biopsies) and nine samples of human kidneys (eight autopsies and one biopsy). The IP staining procedure to detect leptospiral antigen (L. interrogans serovar icterohaemorrhagiae) in human liver and kidney proved to be a reproducible method useful on paraffin embedded tissues after formalin, Bouin's or Helly's fluid fixation. Furthermore, the IP procedure on paraffin-embedded tissue appears to have potential as an aid to the diagnosis. IP stained leptospiral antigen was detected in portal spaces of the liver, engulfed by cells of the mononuclear phagocyte system, in the interstitium of the kidney, and lining vessel walls of both liver and kidney. The results suggest that in acute human leptospirosis (L. interrogans serovar icterohaemorrhagiae) the main factors in the pathogenesis of the lesions are related to the presence of organisms and/or their virulence, including products released by lysis. |
| Databáze: | OpenAIRE |
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