Self-Assembled Cationic-Covered Nanoemulsion as A Novel Biocompatible Immunoadjuvant for Antiserum Production Against Tityus serrulatus Scorpion Venom

Autor:	Denise V. Tambourgi, Alianda Maira Cornélio, Ariane F. Lacerda, Manoela Torres-Rêgo, Arnóbio Antônio da Silva-Júnior, Hugo Alexandre Oliveira Rocha, Eryvaldo Sócrates Tabosa do Egito, Arthur S. A. de Medeiros, Matheus F. Fernandes-Pedrosa
Rok vydání:	2020
Předmět:	Tityus serrulatus nanoemulsion adjuvant medicine.medical_treatment lcsh:RS1-441 Pharmaceutical Science Venom 02 engineering and technology Immunoadjuvant Article lcsh:Pharmacy and materia medica 03 medical and health sciences In vivo Zeta potential medicine liquid crystal Cytotoxicity surface functionalization 030304 developmental biology 0303 health sciences Chromatography biology Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Tityus serrulatus antiserum phase transition immunoadjuvant 0210 nano-technology Cell culture assays Adjuvant
Zdroj:	Pharmaceutics Volume 12 Issue 10 Pharmaceutics, Vol 12, Iss 927, p 927 (2020)
ISSN:	1999-4923
DOI:	10.3390/pharmaceutics12100927
Popis:	This study assesses the efficacy of different nanoemulsion formulations as new and innovative adjuvants for improving the in vivo immunization against the Tityus serrulatus scorpion venom. Nanoemulsions were designed testing key-variables such as surfactants, co-solvents, and the influence of the temperature, which would be able to induce the phase transition from a liquid crystal to a stable nanoemulsion, assessed for four months. Additionally, cationic-covered nanoemulsion with hyper-branched poly(ethyleneimine) was prepared and its performance was compared to the non-cationic ones. The physicochemical properties of the selected nanoemulsions and the interactions among their involved formulation compounds were carefully monitored. The cytotoxicity studies in murine macrophages (RAW 264.7) and red blood cells were used to compare different formulations. Moreover, the performance of the nanoemulsion systems as biocompatible adjuvants was evaluated using mice immunization protocol. The FTIR shifts and the zeta potential changes (from &minus 18.3 ± 1.0 to + 8.4 ± 1.4) corroborated with the expected supramolecular anchoring of venom proteins on the surface of the nanoemulsion droplets. Cell culture assays demonstrated the non-toxicity of the formulations at concentrations less than 1.0 mg/mL, which were able to inhibit the hemolytic effect of the scorpion venom. The cationic-covered nanoemulsion has shown superior adjuvant activity, revealing the highest IgG titer in the immunized animals compared to both the non-cationic counterpart and the traditional aluminum adjuvant. In this approach, we demonstrate the incredible potential application of nanoemulsions as adjuvants, using a nanotechnology platform for antigen delivery system on immune cells. Additionally, the functionalization with hyper-branched poly(ethyleneimine) enhances this recognition and improves its action in immunization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f9eec9eb3df96400cfcc5d5a5cb6d68 https://doi.org/10.3390/pharmaceutics12100927 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.