Occurrence and characteristics of cytotoxic necrotizing factors, cytolethal distending toxins and other virulence factors in Escherichia coli from human blood and faecal samples
| Autor: | H. J. Kadhum, D. Finlay, M.T. Rowe, Ian G. Wilson, H. J. Ball |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
DNA Bacterial Cytolethal distending toxin Epidemiology Virulence Factors Bacterial Toxins Virulence Biology medicine.disease_cause Polymerase Chain Reaction Pilus Microbiology Feces Hemolysin Proteins Plasmid medicine Escherichia coli Humans Adhesins Bacterial Escherichia coli Proteins Hemolysin Chromosomes Bacterial Middle Aged biology.organism_classification Enterobacteriaceae Original Papers Bacterial adhesin Infectious Diseases Blood Fimbriae Bacterial HeLa Cells Plasmids |
| Zdroj: | Epidemiology and infection. 136(6) |
| ISSN: | 0950-2688 |
| Popis: | SUMMARYEscherichia coliisolates from human blood (n=266) and faecal (n=237) samples were examined for cytotoxic necrotizing factors 1 and 2 (CNF 1 and 2), cytolethal distending toxin (CDT), and putative virulence factors that have been associated with disease conditions in humans and animals. PCR showed that the chromosomally encoded, Rho-activating,CNF1(68/544, 12·5%) was more common than the transmissible plasmid-borneCNF2(3/544, 0·6%). The relative risk of having either CNF or CDT toxin genes in blood compared to faecal isolates was 3·88 (95% CI 2·36–6·38). This was highly significant (PE. colibearingCNF1and 11 of 21 (52%) ofE. colibearingCDTalso carried the pyelonephritis-associated pilus gene,papG. The S fimbrial adhesin gene,sfa, was found in 57 blood (21%) and eight faecal samples (3%). The F17 fimbrial adhesin gene and afimbrial adhesin geneafadid not occur frequently. Haemolysin (hly) was found in all of the isolates tested. Further studies must be designed to identify the clinical significance of these genes and their role in pathogenesis. |
| Databáze: | OpenAIRE |
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