The ectodomain of the viral receptor YueB forms a fiber that triggers ejection of bacteriophage SPP1 DNA
| Autor: | Paulo Tavares, Rudi Lurz, Ronald Melki, Carlos São-José, Jean Lepault, Sophie Lhuillier, Mário A. Santos |
|---|---|
| Přispěvatelé: | Unidade Retrovirus & Infeccoes Associadas, Ctr Patogenese Mol, Fac Farm, University of Lisbon, Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Max-Planck-Institut für Molekulare Genetik (MPIMG), Max-Planck-Gesellschaft, Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), Centre National de la Recherche Scientifique (CNRS), Universidade de Lisboa (ULISBOA) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
MECHANISM
[SDV]Life Sciences [q-bio] bacillus subtillis Bacillus Phages Biochemistry Nerve Fibers Myelinated Cell membrane chemistry.chemical_compound COILED-COIL STRUCTURE MESH: Protein Structure Tertiary CELL-WALL bactérie Host cell surface 0303 health sciences Virus receptor Cell biology MESH: Nerve Fibers Myelinated medicine.anatomical_structure Ectodomain protéine Host cell cytoplasm BACILLUS-SUBTILIS 168 PHAGE RESOLUTION TRANSPORT RELEASE Receptors Virus Dimerization Bacillus subtilis MESH: Computational Biology Biology 03 medical and health sciences MESH: Bacillus Phages medicine Molecular Biology 030304 developmental biology 030306 microbiology Computational Biology Cell Biology MESH: Bacillus subtilis Molecular biology MESH: Receptors Virus Protein Structure Tertiary MESH: DNA Viral chemistry MESH: Dimerization adsorption Viral Receptor DNA Viral Peptidoglycan DNA |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281 (17), pp.11464-70. ⟨10.1074/jbc.M513625200⟩ Journal of Biological Chemistry 17 (281), 11464-11470. (2006) |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M513625200⟩ |
| Popis: | International audience; The irreversible binding of bacteriophages to their receptor(s) in the host cell surface triggers release of the naked genome from the virion followed by transit of viral DNA to the host cell cytoplasm. We have purified, for the first time, a receptor from a Gram-positive bacterium that is active to trigger viral DNA ejection in vitro. This extracellular region ("ectodomain") of the Bacillus subtilis protein YueB (YueB780) was a 7 S elongated dimer forming a 36.5-nm-long fiber. YueB780 bound to the tail tip of bacteriophage SPP1. Although a stable receptor-phage interaction occurred between 0 and 37 degrees C, complete blocking of phage DNA release or partial ejection events were observed at temperatures below 15 degrees C. We also showed that the receptor was exposed to the B. subtilis surface. YueB differed structurally from phage receptors from Gram-negative bacteria. Its properties revealed a fiber spanning the full length of the 30-nm-thick peptidoglycan layer. The fiber is predicted to be anchored in the cell membrane through transmembrane segments. These features, highly suitable for a virus receptor in Gram-positive bacteria, are very likely shared by a large number of phage receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|