Modulation of DNA Repair Systems in Blind Cavefish during Evolution in Constant Darkness
| Autor: | Haiyu Zhao, Sebastian Lungu-Mitea, Thomas Braunbeck, Pietro Negrini, Giuseppe Di Mauro, Daniela Vallone, Tilman Lamparter, Cristiano Bertolucci, Andrea Maria Guarino, Nicholas S. Foulkes, Elena Frigato, Hongju Ma |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Fish Proteins DNA Repair DNA repair Cyprinidae Cavefish General Biochemistry Genetics and Molecular Biology NO Evolution Molecular 03 medical and health sciences Models of DNA evolution evolution Animals D-box Enhancer Photolyase Zebrafish Gene Loss function UV damage 030102 biochemistry & molecular biology biology photoreactivation Darkness biology.organism_classification photolyase Cell biology 030104 developmental biology cavefish DNA repair UV damage cavefish evolution photolyase photoreactivation zebrafish General Agricultural and Biological Sciences |
| Zdroj: | Current biology : CB. 28(20) |
| ISSN: | 1879-0445 |
| Popis: | Summary How the environment shapes the function and evolution of DNA repair systems is poorly understood. In a comparative study using zebrafish and the Somalian blind cavefish, Phreatichthys andruzzii, we reveal that during evolution for millions of years in continuous darkness, photoreactivation DNA repair function has been lost in P. andruzzii. We demonstrate that this loss results in part from loss-of-function mutations in pivotal DNA-repair genes. Specifically, C-terminal truncations in P. andruzzii DASH and 6-4 photolyase render these proteins predominantly cytoplasmic, with consequent loss in their functionality. In addition, we reveal a general absence of light-, UV-, and ROS-induced expression of P. andruzzii DNA-repair genes. This results from a loss of function of the D-box enhancer element, which coordinates and enhances DNA repair in response to sunlight. Our results point to P. andruzzii being the only species described, apart from placental mammals, that lacks the highly evolutionary conserved photoreactivation function. We predict that in the DNA repair systems of P. andruzzii, we may be witnessing the first stages in a process that previously occurred in the ancestors of placental mammals during the Mesozoic era. |
| Databáze: | OpenAIRE |
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