Ageing, differentiation, and gene expression in rat epididymal preadipocytes
| Autor: | James L. Kirkland, Charles H. Hollenberg, Wanda Gillon |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Senescence
Male medicine.medical_specialty Aging Cellular differentiation Adipose tissue Gene Expression Cell Separation Biology Biochemistry Tubulin Internal medicine Gene expression medicine Adipocytes Animals RNA Messenger Molecular Biology Gene Cells Cultured Epididymis Messenger RNA Lipoprotein lipase Stem Cells Glyceraldehyde-3-Phosphate Dehydrogenases Cell Differentiation Cell Biology Actins Rats Inbred F344 Rats Lipoprotein Lipase Endocrinology Ageing |
| Zdroj: | Biochemistry and cell biology = Biochimie et biologie cellulaire. 71(11-12) |
| ISSN: | 0829-8211 |
| Popis: | Ageing results in decreased replicative potential of preadipocytes, as well as reduced capacities for the lipid accumulation and increases in lipogenic enzyme activities during differentiation of preadipocytes into fat cells. To determine whether decreased differentiation is associated with decreased levels of mRNA for differentiation-dependent genes and whether early as well as late components of the differentiation programme are affected by ageing, we measured β-actin, α-tubulin, lipoprotein lipase, and glycerol-3-phosphate dehydrogenase mRNA levels in undifferentiated and differentiated epididymal preadipocytes from 3-, 17-, and 24-month-old Fischer 344 rats. During ageing, diminished differentiation-related changes occurred in mRNAs affected early (actin, tubulin), midway through (lipoprotein lipase), and late (glycerol-3-phosphate dehydrogenase) in the preadipocyte differentiation process. Hence, early as well as late phases of the differentiation programme were affected by ageing. The effects involved changes in gene transcription or mRNA processing. Our results were not consistent with the hypothesis that age-related decreases in replication are caused by an increased tendency for cell differentiation.Key words: senescence, adipocyte precursors, β-actin, α-tubulin, lipoprotein lipase, glycerol-3-phosphate dehydrogenase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|