A novel antibody overlay technique for two-dimensional immunoelectrophoresis
| Autor: | J.-M. Bastide, J.C. Lebecq, S.L. Salhi |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Antigens
Fungal Immunology Immunoelectrophoresis Antigen-Antibody Reactions chemistry.chemical_compound Antigen Candida albicans medicine Immunology and Allergy Animals Chemical Precipitation Humans Antibodies Fungal Antiserum medicine.diagnostic_test biology Chemistry Immune Sera Two-Dimensional Immunoelectrophoresis Molecular biology Electrophoresis biology.protein Agarose Somatic antigen Rabbits Antibody Immunoelectrophoresis Two-Dimensional |
| Zdroj: | Journal of immunological methods. 66(2) |
| ISSN: | 0022-1759 |
| Popis: | A new antibody overlay technique for 2-dimensional immunoelectrophoresis (2-D-IEP) is described. After the first dimension electrophoresis of the antigen, the desired amount of antiserum is applied to the initial agarose layer and then evenly distributed over a defined surface of the gel with a PVC film. This modification of the conventional 2-D-IEP procedure makes it possible to perform tandem 2-D-IEPs comparing 2 antisera on the same gel plate, rocket IEPs where several antisera are compared by electrophoresis on the same gel plate, and 2-D-IEPs with an intermediate antiserum, avoiding the need to pour an intermediate gel. With this technique, 77 antigens have been demonstrated in a Candida albicans serotype A somatic antigen preparation. This is the first description of such a large number of immunoprecipitates on the same immunoplate. |
| Databáze: | OpenAIRE |
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