Effects of Mild and Moderate Monoclonal Antibody Dose on Inflammation, Bone Loss, and Activation of the Central Nervous System in a Female Collagen Antibody-induced Arthritis Mouse Model
| Autor: | Mark R. Hutchinson, Egon Perilli, Bonnie Williams, F Lees, Helen Tsangari, Tania N. Crotti |
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| Rok vydání: | 2021 |
| Předmět: |
Cartilage
Articular Lipopolysaccharides Pathology medicine.medical_specialty Histology Lipopolysaccharide medicine.drug_class Inflammatory arthritis Central nervous system Arthritis Osteoclasts Inflammation Monoclonal antibody Severity of Illness Index Arthritis Rheumatoid 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine 030202 anesthesiology Osteoclast Forelimb medicine Animals Edema Periaqueductal Gray Bone Resorption Mice Inbred BALB C business.industry Histocytochemistry Soft tissue Antibodies Monoclonal Articles medicine.disease Arthritis Experimental Disease Models Animal medicine.anatomical_structure chemistry Spinal Cord Female Anatomy medicine.symptom business Neuroglia 030217 neurology & neurosurgery |
| Zdroj: | J Histochem Cytochem |
| ISSN: | 1551-5044 |
| Popis: | Induction of severe inflammatory arthritis in the collagen antibody-induced arthritis (CAIA) murine model causes extensive joint damage and pain-like behavior compromising analysis. While mild models are less severe, their reduced, variable penetrance makes assessment of treatment efficacy difficult. This study aimed to compare macroscopic and microscopic changes in the paws, along with central nervous system activation between a mild and moderate CAIA model. Balb/c mice ( n=18) were allocated to control, mild, and moderate CAIA groups. Paw inflammation, bone volume (BV), and paw volume (PV) were assessed. Histologically, the front paws were assessed for joint inflammation, cartilage damage, and pre/osteoclast-like cells and the lumbar spinal cord and the periaqueductal gray (PAG) region of the brain for glial reactivity. A moderate CAIA dose induced (1) significantly greater local paw inflammation, inflammatory cell infiltration, and PV; (2) significantly more osteoclast-like cells on the bone surface and within the surrounding soft tissue; and (3) significantly greater glial reactivity within the PAG compared with the mild CAIA model. These findings support the use of a moderate CAIA model (higher dose of monoclonal antibodies with low-dose lipopolysaccharide) to induce more consistent histopathological features, without excessive joint destruction. |
| Databáze: | OpenAIRE |
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