Expression Profiles of Toll-Like Receptors in the Differentiation of an Infection with Borrelia burgdorferi Sensu Lato Spirochetes
| Autor: | Teresa Kokot, Krzysztof Solarz, Małgorzata Muc-Wierzgoń, Aleksander Wierzgoń, Sławomir Dudek, Magdalena Kimsa-Dudek, Urszula Mazurek, Ewa Ziółko |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Immunology Oligonucleotides Biology Borrelia afzelii medicine.disease_cause Microbiology 03 medical and health sciences 0302 clinical medicine Lyme disease Borrelia burgdorferi Group Borrelia parasitic diseases medicine Humans Immunology and Allergy Borrelia burgdorferi Oligonucleotide Array Sequence Analysis Skin Lyme Disease Microarray analysis techniques Toll-Like Receptors Cell Differentiation General Medicine Fibroblasts bacterial infections and mycoses medicine.disease biology.organism_classification Virology 030104 developmental biology TLR6 Significance analysis of microarrays Borrelia garinii Transcriptome 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Archivum Immunologiae et Therapiae Experimentalis. 65:175-182 |
| ISSN: | 1661-4917 0004-069X |
| DOI: | 10.1007/s00005-016-0416-8 |
| Popis: | The similarity of Lyme borreliosis to other diseases and its complex pathogenesis present diagnostic and therapeutic difficulties. The changes that occur at the cellular and molecular levels after a Borrelia sp. infection still remain poorly understood. Therefore, the present study focused on the expression of TLR and TLR-signaling genes in human dermal fibroblasts in the differentiation of an infection with Borrelia burgdorferi sensu lato spirochetes. Normal human dermal fibroblasts were cultured with the spirochetes of Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii. Total RNA was extracted from the cells using TRIzol reagent. The analysis of the expression profiles of TLRs and TLR-related genes was performed using commercially available oligonucleotide microarrays of HG-U133A. The GeneSpring 12.0 platform and significance analysis of microarrays were used for the statistical analysis of microarray data. The analyses using the oligonucleotide microarray and QRT-PCR techniques permitted to identify the genes encoding TLR4 and TLR6 as specific for infection with B. afzelii and B. burgdorferi sensu stricto. In turn, TLR3 was only characteristic for an infection with B. burgdorferi sensu stricto. There were no changes in the TLR gene expression after infection with B. garinii. Our findings confirm that Borrelia has a major effect on fibroblast gene expression. Further characterization of changes in gene expression may lead to valuable insights into the role of the toll-like receptor in the pathogenesis of Lyme disease and may provide guidelines for the development of diagnostic markers for an infection with a particular Borrelia genospecies. Moreover, this will help to identify better treatment strategies for Lyme disease. |
| Databáze: | OpenAIRE |
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