nNOS expression in the brain of rats after burn and the effect of the ACE inhibitor captopril
| Autor: | Ibrahim Yaman Saglam, Ebru Demiralay, Esra Sağlam, Göksel Şener, Ahmet Ozer Sehirli, Emine Nur Ozdamar |
|---|---|
| Přispěvatelé: | Maltepe Üniversitesi |
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Captopril nNOS Angiotensin-Converting Enzyme Inhibitors Burn Endogeny Nitric Oxide Synthase Type I Striatum Hippocampal formation Critical Care and Intensive Care Medicine Midbrain ACE inhibitor Internal medicine medicine Animals Rats Wistar business.industry Brain General Medicine Immunohistochemistry Pathophysiology Rats body regions Disease Models Animal Endocrinology nervous system Anesthesia cardiovascular system Emergency Medicine Rat Surgery Burns business Injections Intraperitoneal medicine.drug |
| Zdroj: | Burns. 39:897-904 |
| ISSN: | 0305-4179 |
| Popis: | WOS: 000321602800010 PubMed ID: 23137627 Objective: To investigate the role of endogenous neuronal nitric oxide synthase (nNOS) on brain injury after burn and the effects of the captopril. Methods: Wistar albino rats (200-250 g) were exposed on the dorsal surface to 90 degrees C (burn) or 25 degrees C (sham) water for 10 s. The ACE group was treated with intraperitoneal 10 mg/kg captopril immediately after burn and this treatment was repeated twice daily. At the end of the 24 h brain samples were taken. nNOS was studied in brain areas by immunohistochemistry. Results: There was no difference between the cerebellar and hypothalamic areas the nNOS expression of all groups. nNOS expression increased in the frontal cortex, striatum and midbrain in the burn group compared to the control group. In the frontal cortex, nNOS expression significantly decreased after ACE inhibitor treatment (p < 0.05). The striatal nNOS of the ACE group significantly increased when compared to the control group (p = 0.001). In the midbrain of the animals, nNOS decreased in the ACE group. Hippocampal nNOS expression did not change after burn and significantly increased after ACE inhibitor therapy (p < 0.05). Conclusions: Our data showed that the pathophysiological events following burn appear to be related to an acute inflammatory reaction which is associated with nNOS in the frontal cortex, striatum and midbrain, and captopril treatment abrogates the nNOS response in the frontal cortex and midbrain. (C) 2012 Elsevier Ltd and ISBI. All rights reserved. |
| Databáze: | OpenAIRE |
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