ErbB2 promotes endothelial phenotype of human left ventricular epicardial highly proliferative cells (eHiPC)
| Autor: | Robert S. Kramer, Sarah M. Peterson, Sergey Ryzhov, Edward Jachimowicz, Douglas B. Sawyer, Rutwik Rath, Michael P. Robich, Calvin P.H. Vary, Amanda J. Favreau-Lessard, Reed D. Quinn, Daniel J. Roberts |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Cell Membrane Permeability Receptor ErbB-2 Cellular differentiation Biopsy Heart Ventricles Cell Cell Count 030204 cardiovascular system & hematology Biology Ligands Article 03 medical and health sciences ErbB Receptors 0302 clinical medicine ErbB medicine Animals Humans Receptor skin and connective tissue diseases Molecular Biology Cell Proliferation Epidermal Growth Factor Cell growth Cell Membrane Endothelial Cells Middle Aged Phenotype Rats Up-Regulation 030104 developmental biology medicine.anatomical_structure Cancer research Neuregulin Female Cardiology and Cardiovascular Medicine Pericardium Biomarkers Signal Transduction |
| Zdroj: | Journal of molecular and cellular cardiology. 115 |
| ISSN: | 1095-8584 |
| Popis: | The adult human heart contains a subpopulation of highly proliferative cells. The role of ErbB receptors in these cells has not been studied. From human left ventricular (LV) epicardial biopsies, we isolated highly proliferative cells (eHiPC) to characterize the cell surface expression and function of ErbB receptors in the regulation of cell proliferation and phenotype. We found that human LV eHiPC express all four ErbB receptor subtypes. However, the expression of ErbB receptors varied widely among eHiPC isolated from different subjects. eHiPC with higher cell surface expression of ErbB2 reproduced the phenotype of endothelial cells and were characterized by endothelial cell-like functional properties. We also found that EGF/ErbB1 induces VEGFR2 expression, while ligands for both ErbB1 and ErbB3/4 induce expression of Tie2. The number of CD31posCD45neg endothelial cells is higher in LV biopsies from subjects with high ErbB2 (ErbB2high) eHiPC compared to low ErbB2 (ErbB2low) eHiPC. These findings have important implications for potential strategies to increase the efficacy of cell-based revascularization of the injured heart, through promotion of an endothelial phenotype in cardiac highly proliferative cells. |
| Databáze: | OpenAIRE |
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