Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
| Autor: | Helena Keiko Toma, Deiviane Aparecida Calegar, Márcio Neves Bóia, Jéssica Pereira dos Santos, Marli Maria Lima, Taís Ferreira Gomes, Kerla Joeline Lima Monteiro, Filipe Anibal Carvalho-Costa, Beatriz Coronato Nunes |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Water Wells lcsh:QR1-502 Polymerase Chain Reaction lcsh:Microbiology Entamoeba protozoa Feces 0302 clinical medicine fluids and secretions Prevalence Entamoeba moshkovskii 030212 general & internal medicine Child Aged 80 and over entamoebiasis Entamoebiasis biology Articles Middle Aged Droughts epidemiology Female Entamoeba hartmanni Brazil Microbiology (medical) Adult lcsh:Arctic medicine. Tropical medicine Adolescent lcsh:RC955-962 Dispar 030231 tropical medicine Context (language use) parasites Microbiology 03 medical and health sciences Entamoeba histolytica Young Adult parasitic diseases medicine Humans Amoebiasis Poverty Aged Drinking Water DNA Protozoan medicine.disease biology.organism_classification Molecular Typing Cross-Sectional Studies |
| Zdroj: | Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Volume: 111, Issue: 2, Pages: 114-119, Published: 02 FEB 2016 Memórias do Instituto Oswaldo Cruz., Vol 111, Iss 2, Pp 114-119 (2016) |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | This study aimed to estimate the frequency, associated factors, and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, andEntamoeba hartmanni infections. We performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. Faecal samples were processed through flotation and centrifugation methods.E. histolytica, E. dispar, E. moshkovskii, and E. hartmanni were identified by nested-polymerase chain reaction (PCR). The overall prevalence of infection was 22/213 (10.3%). The infection rate among subjects who drink rainwater collected from roofs in tanks was higher than the rate in subjects who drink desalinated water pumped from wells; similarly, the infection rate among subjects who practice open defecation was significantly higher than that of subjects with latrines. Out of the 22 samples positive for morphologically indistinguishableEntamoeba species, the differentiation by PCR was successful for 21. The species distribution was as follows: 57.1% to E. dispar, 23.8% to E. histolytica, 14.3% toE. histolytica and E. dispar, and 4.8% E. dispar and E. hartmanni. These data suggest a high prevalence of asymptomatic infection by the group of morphologically indistinguishable Entamoeba histolytica/dispar/moshkovskiicomplex and E. hartmanni species. In this context of water scarcity, the sanitary and socioenvironmental characteristics of the region appear to favour transmission. |
| Databáze: | OpenAIRE |
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