Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization
| Autor: | Huddee Ho, Hong Tan, Tim Ellis, Pu Li, Juan Gamez, Michael Howes, Bettina Heidecker, Greg L. Carricato, Weilei Ma, Henrik Persson, Scott Lockard, Danfeng Yao, Jing Wei, Robert F. Zuk, Sae Choo, Janette Phi-Wilson, Michael W. Recknor, Lian-She Zhao, Ram Varma, Kevin Du, Joy Concepcion, Donald Perillat, Krista Witte, Charles Wartchow |
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| Rok vydání: | 2009 |
| Předmět: |
Analyte
Time Factors Staining and Labeling Chemistry Antigen-antibody reactions Organic Chemistry technology industry and agriculture Proteins Nanotechnology Biosensing Techniques macromolecular substances General Medicine Ligands Antibodies Computer Science Applications Characterization (materials science) Antigen-Antibody Reactions Kinetics Interferometry Drug Discovery Biosensor Label free |
| Zdroj: | Combinatorial Chemistry & High Throughput Screening. 12:791-800 |
| ISSN: | 1386-2073 |
| DOI: | 10.2174/138620709789104915 |
| Popis: | The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters. |
| Databáze: | OpenAIRE |
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