DNA damage after exhaustive treadmill running in trained and untrained men
| Autor: | Bertram Poch, M Grünert-Fuchs, A Hartmann, A. M. Niess, G Speit |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Electrophoresis Male medicine.medical_specialty DNA damage Physical Therapy Sports Therapy and Rehabilitation medicine.disease_cause Running Lipid peroxidation chemistry.chemical_compound Treadmill running Internal medicine Malondialdehyde medicine Humans Orthopedics and Sports Medicine Treadmill Gel electrophoresis business.industry Plasma levels Adaptation Physiological Oxidative Stress Endocrinology chemistry Physical therapy Exercise Test business Oxidative stress DNA Damage |
| Zdroj: | International journal of sports medicine. 17(6) |
| ISSN: | 0172-4622 |
| Popis: | The single cell gel electrophoresis (SCG) assay was used to compare the occurrence of DNA damage in peripheral white blood cells in 6 trained (TR) and 5 untrained (UT) men after exhaustive exercise. The subjects completed an incremental treadmill test until exhaustion (maximal lactate: 12.9 +/- 1.7 in TR and 12.2 +/- 2.5 mmol.l-1 in UT). A clear and significant increase of DNA migration from 2.31 +/- 0.20 (TR) and 2.22 +/- 0.16 (UT) at rest to 2.65 +/- 0.30 (TR) and 3.00 +/- 0.41 tail moment (UT) was found 24 hours after exercise. Noteworthy is that the increase of DNA migration was significantly lower in TR (+ 18.7 +/- 6.8%) compared to UT (+ 35.7 +/- 8.9%). Plasma levels of malondialdehyde (MDA) were not significantly increased in TR and UT after exercise. At rest and 15 minutes after exercise MDA-values were significantly lower in TR compared to UT. In conclusion the present investigation demonstrates the occurrence of DNA damage in white blood cells following exhaustive exercise. This observation may be induced by oxidative stress. Our data suggest that adaptation to training seems to be capable of reducing free radical associated effects, such as DNA damage. Further investigations are needed to clarify the causal mechanisms and biological relevance of exercise-induced DNA damage. |
| Databáze: | OpenAIRE |
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