In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines
| Autor: | Rajendra B Nerli, Vijay M. Kumbar, Murigendra B Hiremath, Shridhar C Ghagane, R.H. Aladakatti, S. E. Neelagund, Sridevi I. Puranik, Sunil Jalalpure |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
antioxidant Antioxidant medicine.medical_treatment Ethyl acetate phytochemical anticancer Anthraquinone 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine MTT assay Phenols lcsh:Miscellaneous systems and treatments chemistry.chemical_classification Leea indica biology Traditional medicine Glycoside prostate cancer lcsh:RZ409.7-999 biology.organism_classification 030104 developmental biology Complementary and alternative medicine chemistry Phytochemical Biochemistry 030220 oncology & carcinogenesis Original Article |
| Zdroj: | Integrative Medicine Research, Vol 6, Iss 1, Pp 79-87 (2017) Integrative Medicine Research |
| ISSN: | 2213-4220 |
| DOI: | 10.1016/j.imr.2017.01.004 |
| Popis: | Background To determine the phytochemical constituents, antioxidant, and anticancer activities of Leea indica leaf extracts on DU-145 and PC-3 human prostate cancer cell lines. Methods Leaf sample was subjected to Soxhlet extraction method with increasing polarity of solvents, namely, chloroform, ethyl acetate, methanol, ethanol, and aqueous. Phytochemical screening was done using different biochemical tests. Quantitative analysis for phenol was determined by Folin–Ciocalteu reagent method. The antioxidant activity was tested using 2,2-diphenyl-1-picrylhydrazyl, ferric ion reducing power assay, and phosphomolybdenum assay. In vitro anticancer activity on DU-145 and PC-3 human prostate cancer cell lines was evaluated by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assay. Results Phytochemical screening confirmed the presence of phyto-constituents like alkaloids, flavonoids, glycosides, phenols, lignins, saponins, sterols, tannins, anthraquinone, and reducing sugar. Methanol and ethanol extracts exhibited higher phenolic content as compare to aqueous extract. Antioxidant capacities were shown highest in methanol and ethanol extracts based on the test performed. The methanol and ethanol leaf extracts were found to be selectively cytotoxic in vitro to (DU-145 and PC-3) prostate cancer cell lines with IC 50 values 529.44±42.07μg/mL and 677.11±37.01μg/mL for DU-145 and 547.55±33.52μg/mL and 631.99±50.24μg/mL for PC-3 respectively, while it had no cytotoxic effect on normal mice embryo fibroblast cells. Conclusion The results indicate that Leea indica was a promising antioxidant and anticancer agent for DU-145 and PC-3 human prostate cancer cell lines. However, further studies are needed to conclude its therapeutic use. |
| Databáze: | OpenAIRE |
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