Prometastatic GPCR CD97 Is a Direct Target of Tumor Suppressor microRNA-126
| Autor: | Ying Y. Lu, David A. Tirrell, Michael J. Sweredoski, David Huss, Sonja Hess, Rusty Lansford |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Breast Neoplasms Biology Biochemistry Article Receptors G-Protein-Coupled law.invention Downregulation and upregulation Antigens CD law Stable isotope labeling by amino acids in cell culture microRNA medicine Humans Neoplasm Invasiveness Breast Cancer General Medicine medicine.disease Metastatic breast cancer Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs Cancer cell Cancer research Molecular Medicine Suppressor Female |
| Zdroj: | ACS Chemical Biology. 9:334-338 |
| ISSN: | 1554-8937 1554-8929 |
| DOI: | 10.1021/cb400704n |
| Popis: | Tumor suppressor microRNA-126 (miR-126) is often down-regulated in cancer cells, and its over-expression is found to inhibit cancer metastasis. To elucidate the mechanism of tumor suppression by miR-126, we analyzed the proteomic response to miR-126 over-expression in the human metastatic breast cancer cell line MDA-MB-231. To acquire quantitative, time-resolved information, we combined two complementary proteomic methods, BONCAT and SILAC. We discovered a new direct target of miR-126: CD97, a pro-metastatic G-protein-coupled receptor (GPCR) that has been reported to promote tumor cell invasion, endothelial cell migration and tumor angiogenesis. This discovery establishes a link between down-regulation of miR-126 and over-expression of CD97 in cancer, and provides new mechanistic insight into the role of miR-126 in inhibiting both cell-autonomous and non-cell-autonomous cancer progression. |
| Databáze: | OpenAIRE |
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