New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças
| Autor: | Kumi Shiota Ozawa, Tatiane Melina Guerreiro, Estela de Oliveira Lima, Carlos Fernando Odir Rodrigues Melo, Rodrigo Ramos Catharino, Diogo Noin de Oliveira, Simone Pereira do Nascimento Triano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
QuEChERS
alcoholic beverages Endocrinology Diabetes and Metabolism lcsh:TX341-641 ethyl carbamate Quechers 01 natural sciences law.invention chemistry.chemical_compound 0404 agricultural biotechnology law gas chromatography–mass spectrometry Sugar Distillation Nutrition Original Research Nutrition and Dietetics Chromatography 010401 analytical chemistry Extraction (chemistry) 04 agricultural and veterinary sciences cachaça 040401 food science 0104 chemical sciences Triple quadrupole mass spectrometer chemistry Ethyl carbamate Gas chromatography Gas chromatography–mass spectrometry lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Frontiers in Nutrition Frontiers in Nutrition, Vol 5 (2018) |
| ISSN: | 2296-861X |
| Popis: | Cachaça is a popular spirit produced in Brazil, obtained by distillation of fermented sugar cane. Among the contaminants arising from production, ethyl carbamate is a carcinogenic compound that occurs naturally in fermented foods and beverages; in Brazil, the maximum limit established by current legislation is 150 µg L−1. Quality control is usually performed using gas chromatography; however, robustness and reproducibility of quantitative results may be severely impaired, as the addition of 6–30 g L−1 of sucrose is a common procedure for taste standardization, directly interfering in the results. This work describes the development of a novel method to improve ethyl carbamate quantification in cachaças using a new approach of QuEChERS extraction based on salting-out phenomenon, to effectively separate ethanol from sugar-containing water. Eighteen different brands of cachaça were analyzed. The proposed methodology was able to eliminate components that contaminate the sample flow path in the gas chromatography system, while improving precision and accuracy by using a triple-quadrupole approach, in comparison with the methodology usually employed: direct analysis of cachaça samples with no sample prep. Results indicate that this approach is more effective due to the removal of sugar content, with no impact in costs per analysis. |
| Databáze: | OpenAIRE |
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