Need for Methods to Investigate Endocannabinoid Signaling
| Autor: | Mauro Maccarrone |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
0301 basic medicine
Cannabinoid receptor Metabolic routes 2-Arachidonoylglycerol Biology Signal transduction Immunochemical assays 03 medical and health sciences chemistry.chemical_compound Anandamide Enzyme assays Intracellular trafficking Localization Oxidative pathways Receptor binding assays Cannabinoid Receptor Agonists Humans Receptors Cannabinoid Signal Transduction Dronabinol Endocannabinoids Receptors medicine Receptor Tetrahydrocannabinol Cannabinoid Endocannabinoid system Cell biology 030104 developmental biology chemistry Metabolic enzymes Neuroscience medicine.drug |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071627273 Methods in Molecular Biology ISBN: 9781493935376 |
| Popis: | Endocannabinoids (eCBs) are endogenous lipids able to activate cannabinoid receptors, the primary molecular targets of the cannabis (Cannabis sativa) active principle Δ(9)-tetrahydrocannabinol. During the last 20 years, several N-acylethanolamines and acylesters have been shown to act as eCBs, and a complex array of receptors, metabolic enzymes, and transporters (that altogether form the so-called eCB system) has been shown to finely tune their manifold biological activities. It appears now urgent to develop methods and protocols that allow to assay in a specific and quantitative manner the distinct components of the eCB system, and that can properly localize them within the cell. A brief overview of eCBs and of the proteins that bind, transport, and metabolize these lipids is presented here, in order to put in a better perspective the relevance of methodologies that help to disclose molecular details of eCB signaling in health and disease. Proper methodological approaches form also the basis for a more rationale and effective drug design and therapeutic strategy to combat human disorders. |
| Databáze: | OpenAIRE |
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