The uncoupling of respiratory-chain phosphorylation by 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole
| Autor: | Beechey Rb |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
Oligomycin
Antimetabolites Manometry Uncoupling Agents General Mathematics In Vitro Techniques Mitochondrion Guanidines Oxidative Phosphorylation chemistry.chemical_compound Respiration Animals Submitochondrial particle Adenosine Triphosphatases Chemistry Myocardium Applied Mathematics Articles Mitochondria Rats Biochemistry Phosphorylation Benzimidazoles Cattle Oligomycins Chromatography Thin Layer NAD+ kinase Respiration rate |
| Zdroj: | Biochemical Journal. 98:284-289 |
| ISSN: | 0006-2936 |
| DOI: | 10.1042/bj0980284 |
| Popis: | 1. 30mμm-4,5,6,7-Tetrachloro-2-trifluoromethylbenzimidazole (TTFB) uncouples respiratory-chain phosphorylation. The respiration rate of uncoupled mitochondria is significantly greater than the state 3 rate. Neither added orthophosphate nor ATP is required to sustain this rate. The oligomycin- and octylguanidine-induced inhibitions of respiration are relieved by uncoupling concentrations of TTFB. 2. Uncoupling concentrations of TTFB elicit a high adenosinetriphosphatase activity and inhibit the ATP-dependent succinate-linked reduction of NAD catalysed by submitochondrial particles from ox-heart mitochondria. 3. The action of TTFB is similar to that of 2,4-dinitrophenol. Evidence is presented which shows that it is the anionic forms of benzimidazoles and imidazoles, having the negative charge localized on the ring nitrogen atom, which are the effective uncoupling agents. |
| Databáze: | OpenAIRE |
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