Fetal exposure to diesel exhaust affects X-chromosome inactivation factor expression in mice
| Autor: | Hirohisa Takano, Shigeru Oshio, Ken Takeda, Takayuki Kumamoto, Naomi Tsukue |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Microarray Biology Real-Time Polymerase Chain Reaction Toxicology X-inactivation MECP2 Mice Pregnancy X Chromosome Inactivation Gene expression Animals RNA Messenger Maternal-Fetal Exchange Gene Vehicle Emissions Inhalation Exposure Mice Inbred ICR Dose-Response Relationship Drug Effector Gene Expression Profiling Brain Gene Expression Regulation Developmental Cell biology Maternal Exposure Tissue Array Analysis Prenatal Exposure Delayed Effects Female RNA Long Noncoding XIST Tsix |
| Zdroj: | The Journal of Toxicological Sciences. 38:245-254 |
| ISSN: | 1880-3989 0388-1350 |
| DOI: | 10.2131/jts.38.245 |
| Popis: | Several studies have shown effects of diesel exhaust (DE) on the central nervous system, but the mechanism is unclear. Fetal mice were exposed to whole DE (contains gases and particles) in an inhalation chamber, and cerebrum gene expression changes were examined by gene assay (microarray and quantitative real-time PCR). By microarray, upregulation of Xist, B-raf and Drwms2 were detected. Especially, mRNA expression of Xist was increased in a concentration-dependent manner in male and female mice. Xist (X-inactive specific transcript) is a major effector of the X-inactivation process, and X-linked genes are highly expressed in brain tissue and consistent with a role in brain developments. By quantitative real-time PCR, Tsix (crucial noncoding antisense partner of Xist) and other X-linked genes (Mecp2, Hprt1, and Sts) were examined; Tsix was upregulated, and other X-linked genes were unaffected in the male and female mice. Our findings suggest that exposure to DE increases Xist and Tsix gene expression in utero without influencing X-linked gene expression. An examination of Xist gene expression changes may provide an important biomarker for DE-induced effects. The possibility of avoiding X-chromosome inactivation (XCI) mechanisms by minimizing exposure to DE is expected. |
| Databáze: | OpenAIRE |
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