Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm
| Autor: | Benoît Zuber, Rok Kostanjšek, Kristina Sepčić, Špela Modic, Jaka Razinger, Matej Milijaš Jotić, Graziano Guella, Francesco Trenti, Ioan Iacovache, Anastasija Panevska, Martina Mravinec, Matej Skočaj, Ana Pavšič |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Insecticides MACPF-protein Brush border Health Toxicology and Mutagenesis 610 Medicine & health western corn rootworm Toxicology protein z domeno MACPF Pore forming protein Article Fungal Proteins koruzni hrošč 03 medical and health sciences Hemolysin Proteins 0302 clinical medicine oyster mushroom udc:577 medicine Animals porotvorni protein egerolizin biology Chemistry Vesicle biology.organism_classification Sphingolipid Transmembrane protein bioinsekticid Cell biology Sphingomyelins Coleoptera Gastrointestinal Tract 030104 developmental biology medicine.anatomical_structure Western corn rootworm aegerolysin pore-forming protein Larva bioinsecticide Medicine Basal lamina Sphingomyelin bukov ostrigar 030217 neurology & neurosurgery |
| Zdroj: | Toxins Toxins, Vol 13, Iss 455, p 455 (2021) Toxins, vol. 13, no. 7, 455, 2021. Milijaš Jotić, Matej Milijaš; Panevska, Anastasija; Iacovache, Ioan; Kostanjšek, Rok; Mravinec, Martina; Skočaj, Matej; Zuber, Benoît; Pavšič, Ana; Razinger, Jaka; Modic, Špela; Trenti, Francesco; Guella, Graziano; Sepčić, Kristina (2021). Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm. Toxins, 13(7) MDPI 10.3390/toxins13070455 Volume 13 Issue 7 |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins13070455 |
| Popis: | Ostreolysin A6 (OlyA6) is a protein produced by the oyster mushroom (Pleurotus ostreatus). It binds to membrane sphingomyelin/cholesterol domains, and together with its protein partner, pleurotolysin B (PlyB), it forms 13-meric transmembrane pore complexes. Further, OlyA6 binds 1000 times more strongly to the insect-specific membrane sphingolipid, ceramide phosphoethanolamine (CPE). In concert with PlyB, OlyA6 has potent and selective insecticidal activity against the western corn rootworm. We analysed the histological alterations of the midgut wall columnar epithelium of western corn rootworm larvae fed with OlyA6/PlyB, which showed vacuolisation of the cell cytoplasm, swelling of the apical cell surface into the gut lumen, and delamination of the basal lamina underlying the epithelium. Additionally, cryo-electron microscopy was used to explore the membrane interactions of the OlyA6/PlyB complex using lipid vesicles composed of artificial lipids containing CPE, and western corn rootworm brush border membrane vesicles. Multimeric transmembrane pores were formed in both vesicle preparations, similar to those described for sphingomyelin/cholesterol membranes. These results strongly suggest that the molecular mechanism of insecticidal action of OlyA6/PlyB arises from specific interactions of OlyA6 with CPE, and the consequent formation of transmembrane pores in the insect midgut. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |