Murine APOBEC1 Is a Powerful Mutator of Retroviral and Cellular RNA In Vitro and In Vivo
| Autor: | Denise Guetard, Marc Sitbon, Jean-Pierre Vartanian, Anne Keriel, Myrtille Renard, Simon Wain-Hobson, Vincent Petit |
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| Přispěvatelé: | Rétrovirologie Moléculaire, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) |
| Rok vydání: | 2009 |
| Předmět: |
Hypoxanthine Phosphoribosyltransferase
MESH : Molecular Sequence Data MESH : RNA Messenger MESH : DNA Complementary MESH : NIH 3T3 Cells viruses Muscle Proteins MESH: Base Sequence Nucleic Acid Denaturation MESH : Retroviridae Infections MESH: Animals Newborn Mice chemistry.chemical_compound MESH : Hypoxanthine Phosphoribosyltransferase Structural Biology Murine leukemia virus MESH : RNA MESH: Animals APOBEC Deaminases MESH : Cytidine Deaminase Gammaretrovirus MESH : Muscle Proteins 0303 health sciences biology Nucleotides MESH : Animals Newborn 030302 biochemistry & molecular biology MESH: Leukemia Virus Murine Cytidine Cytidine deaminase MESH: Apolipoproteins B 3. Good health Leukemia Virus Murine MESH: Retroviridae Infections [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH : Mutation MESH: Genome Viral MESH : Genome Viral MESH : Leukemia Virus Murine MESH : Nucleic Acid Denaturation APOBEC DNA Complementary MESH: Mutation APOBEC-1 Deaminase Molecular Sequence Data MESH: Leukemia Experimental Genome Viral MESH: Nucleic Acid Denaturation [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH: Muscle Proteins 03 medical and health sciences MESH: RNA Cytidine Deaminase MESH : Mice MESH : RNA Editing Animals Humans MESH: RNA Editing RNA Messenger MESH: Mice Molecular Biology Apolipoproteins B MESH: RNA Messenger MESH: Cytidine Deaminase 030304 developmental biology Messenger RNA Leukemia Experimental MESH: Molecular Sequence Data MESH: Humans Base Sequence APOBEC1 MESH : Humans MESH: Tumor Virus Infections RNA MESH : Apolipoproteins B MESH: DNA Complementary biology.organism_classification MESH: Hypoxanthine Phosphoribosyltransferase Molecular biology MESH: Nucleotides Tumor Virus Infections Animals Newborn chemistry Mutation NIH 3T3 Cells MESH : Nucleotides MESH : Leukemia Experimental MESH : Base Sequence MESH : Animals RNA Editing MESH : Tumor Virus Infections Retroviridae Infections MESH: NIH 3T3 Cells |
| Zdroj: | Journal of Molecular Biology Journal of Molecular Biology, Elsevier, 2009, 385 (1), pp.65-78. ⟨10.1016/j.jmb.2008.10.043⟩ Journal of Molecular Biology, 2009, 385 (1), pp.65-78. ⟨10.1016/j.jmb.2008.10.043⟩ Journal of Molecular Biology, Elsevier, 2009, 385 (1), pp.65-78. 〈10.1016/j.jmb.2008.10.043〉 |
| ISSN: | 0022-2836 1089-8638 |
| DOI: | 10.1016/j.jmb.2008.10.043 |
| Popis: | International audience; Mammalian APOBEC molecules comprise a large family of cytidine deaminases with specificity for RNA and single-stranded DNA (ssDNA). APOBEC1s are invariably highly specific and edit a single residue in a cellular mRNA, while the cellular targets for APOBEC3s are not clearly established, although they may curtail the transposition of some retrotransposons. Two of the seven member human APOBEC3 enzymes strongly restrict human immunodeficiency virus type 1 in vitro and in vivo. We show here that ssDNA hyperediting of an infectious exogenous gammaretrovirus, the Friend-murine leukemia virus, by murine APOBEC1 and APOBEC3 deaminases occurs in vitro. Murine APOBEC1 was able to hyperdeaminate cytidine residues in murine leukemia virus genomic RNA as well. Analysis of the edited sites shows that the deamination in vivo was due to mouse APOBEC1 rather than APOBEC3. Furthermore, murine APOBEC1 is able to hyperedit its primary substrate in vivo, the apolipoprotein B mRNA, and a variety of heterologous RNAs. In short, murine APOBEC1 is a hypermutator of both RNA and ssDNA in vivo, which could exert occasional side effects upon overexpression. |
| Databáze: | OpenAIRE |
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