Trypanosoma cruzi: Immunological predictors of benznidazole efficacy during experimental infection
| Autor: | María Elisa Solana, María Celia Fernández, Stella M. González Cappa |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Male Ratón T-Lymphocytes Trypanosoma cruzi Immunology Population Antibodies Protozoan Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Parasitemia CD8-Positive T-Lymphocytes Polymerase Chain Reaction Immunophenotyping Mice Immune system Antigens CD parasitic diseases Splenocyte medicine Animals Chagas Disease Lectins C-Type Muscle Skeletal education Mice Inbred C3H education.field_of_study biology General Medicine DNA Protozoan Flow Cytometry biology.organism_classification medicine.disease Trypanocidal Agents CD4 Lymphocyte Count Hyaluronan Receptors Infectious Diseases Nitroimidazoles Benznidazole Immunoglobulin G biology.protein Parasitology Antibody Spleen medicine.drug |
| Zdroj: | Experimental Parasitology. 124:172-180 |
| ISSN: | 0014-4894 |
| Popis: | C3H/HeN male mice were infected with a lethal population of Trypanosoma cruzi and treated with benznidazole (Bz). Parasitemia, body weight and survival rate were registered during the therapy with significant improvement for T. cruzi-infected Bz-treated animals. Besides, flow cytometry resulted a useful method to discriminate between cured animals from those not cured by monitoring IgG(1) bound to live trypomastigotes levels. At the end of Bz therapy, the LT splenocyte compartment was studied for activation/memory cell surface markers (CD(69)(+) and CD(44)(+)). Cytofluorometric analysis showed that T. cruzi-infected untreated mice increased their activated LT numbers and this effect was completely abolished only in cured mice at the end of Bz administration. The same behavior was observed for the memory LT subpopulation correlating to an effector memory (CD(62L)(-)) displayed by T. cruzi infection. Bz treatment was able to modulate the immunological response by reducing the deleterious effect of the acute phase in all T. cruzi-infected mice. |
| Databáze: | OpenAIRE |
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