Investigating the macropinocytic proteome ofDictyosteliumamoebae by high-resolution mass spectrometry
| Autor: | Agnès Chapel, Christophe Bruley, Gérard Klein, Yves Vandenbrouck, Christophe Masselon, Sabine Brugière, Agnès Journet, Laurence Aubry, Sylvie Kieffer |
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| Přispěvatelé: | Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Masselon, Christophe |
| Rok vydání: | 2011 |
| Předmět: |
Proteomics
Proteome Blotting Western Protozoan Proteins [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Endocytosis Biochemistry Mass Spectrometry Dictyostelium discoideum Slime mold Dictyostelium Axenic Molecular Biology ComputingMilieux_MISCELLANEOUS biology Computational Biology [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology biology.organism_classification Cell biology Protein Transport Magnets Pinocytosis Electrophoresis Polyacrylamide Gel Eukaryote Lysosomes Chromatography Liquid |
| Zdroj: | Proteomics Proteomics, Wiley-VCH Verlag, 2012 Proteomics, 2012 HAL |
| ISSN: | 1615-9853 1615-9861 |
| DOI: | 10.1002/pmic.201100313 |
| Popis: | The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis process, we established the inventory of proteins associated with this pathway using mass spectrometry-based proteomics. Using a magnetic purification procedure and high-performance LC-MS/MS proteome analysis, a list of 2108 non-redundant proteins was established, of which 24% featured membrane-spanning domains. Bioinformatics analyses indicated that the most abundant proteins were linked to signaling, vesicular trafficking and the cytoskeleton. The present repertoire validates our purification method and paves the way for a future proteomics approach to study the dynamics of macropinocytosis. |
| Databáze: | OpenAIRE |
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