Elucidating chromatin and nuclear domain architecture with electron spectroscopic imaging
| Autor: | David P. Bazett-Jones, Hesam Dehghani, Rosa Nisman, Eden Fussner, Ren Li |
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| Rok vydání: | 2008 |
| Předmět: |
Nitrogen
Nucleolus law.invention Mice chemistry.chemical_compound Microscopy Electron Transmission law Genetics medicine Animals Humans Chromatin Fiber biology Spectrum Analysis Nuclear Proteins Phosphorus DNA Cell Nucleus Structures Chromatin Cell biology Cell nucleus Histone medicine.anatomical_structure chemistry Transmission electron microscopy biology.protein RNA Electron microscope Cell Nucleolus |
| Zdroj: | Chromosome Research. 16:397-412 |
| ISSN: | 1573-6849 0967-3849 |
| DOI: | 10.1007/s10577-008-1237-3 |
| Popis: | Electron microscopy has been the 'gold standard' of spatial resolution for studying the structure of the cell nucleus. Electron spectroscopic imaging (ESI) offers advantages over conventional transmission electron microscopy by eliminating the need for heavy-atom contrast agents. ESI also provides mass-dependent and element-specific information at high resolution, permitting the distinguishing of structures that are primarily composed of protein, DNA, or RNA. The technique can be applied to understand the structural consequences of epigenetic modifications, such as modified histones, on chromatin fiber morphology. ESI can also be applied to elucidate the multifunctional behavior of subnuclear 'organelles' such as the nucleolus and promyelocytic leukemia nuclear bodies. |
| Databáze: | OpenAIRE |
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