Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases
| Autor: | Pilar Navarro, Cristina Muñoz-San Martín, Susana Campuzano, Maria Gamella, José M. Pingarrón, María Pedrero, Pablo García de Frutos, Rebeca M. Torrente-Rodríguez, Neus Martínez-Bosch |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Comunidad de Madrid, Instituto de Salud Carlos III, European Commission |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
human ST2
Streptavidin pancreatic cancer Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Biosensing Techniques 030204 cardiovascular system & hematology Article Antibodies Oncología Plasma Magnetics 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Limit of Detection Neoplasms Humans Electrodes plasma 030304 developmental biology Immunoassay Detection limit 0303 health sciences Chromatography biology Pancreatic cancer Electrochemical Techniques Hydrogen Peroxide General Medicine Primary and secondary antibodies Carbon Amperometry electrochemical immune platform Human ST2 chemistry Biotinylation biology.protein Electrochemical immune platform Target protein TP248.13-248.65 Biotechnology Conjugate Peroxidase |
| Zdroj: | Biosensors, Vol 11, Iss 202, p 202 (2021) Biosensors Volume 11 Issue 6 E-Prints Complutense. Archivo Institucional de la UCM instname Digital.CSIC. Repositorio Institucional del CSIC |
| Popis: | A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates built on the surface of carboxylic acid-microsized magnetic particles (HOOC-MBs) were used to selectively capture ST2. A biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP) was used to accomplish the sandwiching of the target protein. The immune platform exhibits great selectivity and a low limit of detection (39.6 pg mL ) for ST2, allowing the determination of soluble ST2 (sST2) in plasma samples from healthy individuals and patients diagnosed with pancreatic ductal adenocarcinoma (PDAC) in only 45 min once the immunoconjugates have been prepared. The good correlation of the obtained results with those provided by an ELISA kit performed using the same immunoreagents demonstrates the potential of the developed strategy for early diagnosis and/or prognosis of the fatal PDAC disease. This research was funded by Spanish Ministerio de Ciencia e Innovación. PID2019- 103899RB-I00, Spanish Ministerio de Ciencia, Innovación y Universidades, RTI2018-095672-B-I00, Comunidad de Madrid, S2018/NMT-4349, and AES-ISCIII/FEDER, PI20/00625 |
| Databáze: | OpenAIRE |
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