Special biochemical responses to nitrogen deprivation of filamentous oleaginous microalgae Tribonema sp
| Autor: | Junfeng Wang, Fajin Guo, Dong Qingzhe, Lili Gao, Hui Wang, Zhou Wenjun, Tianzhong Liu, Wei Zhang, Lin Chen |
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| Rok vydání: | 2014 |
| Předmět: |
Tribonema
Environmental Engineering Nitrogen Starch chemistry.chemical_element Bioengineering Biology chemistry.chemical_compound Microscopy Electron Transmission Dry weight Botany Biomass Food science Waste Management and Disposal Triglycerides chemistry.chemical_classification Renewable Energy Sustainability and the Environment Fatty Acids Fatty acid General Medicine Carbohydrate biology.organism_classification Culture Media chemistry Biodiesel production Stramenopiles Nannochloropsis |
| Zdroj: | Bioresource Technology. 158:19-24 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2014.01.144 |
| Popis: | Both filamentous microalgae Tribonema and unicellular microalgae Nanochloropsis are promising feedstock for biodiesel production. Nitrogen starvation increased lipid content in Nannochloropsis but decreased that in Tribonema. In this study, biochemical responses of Tribonema under different levels of nitrogen (0N, 0.05N, 0.1N and 1N-BG11) were investigated. 1N-BG11 was sufficient during 15-day-cultivation, while the other levels were nitrogen limited. Cell growth was interrupted with 0N-BG11, but no differences in biomass among 0.05N, 0.1N and 1N-BG11. Both protein and lipid contents (% of dry weight) declined gradually inversely to the increment in carbohydrate contents under the decreasement of nitrogen levels. Both assays and TEM results showed that the cytoplasm in Tribonema contained no starch. Compared to nitrogen-replete condition, the TAG content (% of dry weight) decreased obviously under nitrogen starvation. Different levels of nitrogen did not cause fundamental shifts in fatty acid profiles in Tribonema. |
| Databáze: | OpenAIRE |
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