Dietary fish oil reduces glomerular injury and elevated renal hydroxyeicosatetraenoic acid levels in the JCR:LA-cprat, a model of the metabolic syndrome
| Autor: | Harold M. Aukema, Spencer D. Proctor, Jing Lu, Faye Borthwick |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Genotype Kidney Glomerulus Medicine (miscellaneous) 6-Ketoprostaglandin F1 alpha Biology Dinoprost chemistry.chemical_compound Fish Oils Internal medicine Hydroxyeicosatetraenoic Acids medicine Albuminuria Animals Metabolic Syndrome chemistry.chemical_classification Kidney Nutrition and Dietetics Prostaglandin D2 Eicosanoid metabolism Glomerulosclerosis Rats Inbred Strains Fish oil medicine.disease Dietary Fats Rats Thromboxane B2 Disease Models Animal Endocrinology medicine.anatomical_structure chemistry Dietary Supplements Prostaglandins Kidney Diseases lipids (amino acids peptides and proteins) medicine.symptom Metabolic syndrome Polyunsaturated fatty acid |
| Zdroj: | British Journal of Nutrition. 110:11-19 |
| ISSN: | 1475-2662 0007-1145 |
| Popis: | We have previously shown nutritional intervention with fish oil (n-3 PUFA) to reduce numerous complications associated with the metabolic syndrome (MetS) in the JCR:LA-corpulent (cp) rat. In the present study, we sought to explore the potential role of fish oil to prevent glomerulosclerosis in JCR:LA-cprats via renal eicosanoid metabolism and lipidomic analysis. Male lean and MetS JCR:LA-cprats were fed a lipid-balanced diet supplemented with fish oil (5 or 10 % of total fat). After 16 weeks of feeding, albuminuria was significantly reduced in MetS rats supplemented with 5 or 10 % fish oil ( − 53 and − 70 %, respectively, compared with the untreated MetS rats). The 5 % fish oil diet resulted in markedly lower glomerulosclerosis ( − 43 %) in MetS rats and to a lesser extent in those supplemented with 10 % fish oil. Interestingly, untreated MetS rats had higher levels of 11- and 12-hydroxyeicosatetraenoic acids (HETE)v.lean rats. Dietary fish oil reduced these levels, as well as other (5-, 9- and 15-) HETE. Whilst genotype did not alter prostanoid levels, fish oil reduced endogenous renal levels of 6-keto PGF1α(PGI2metabolite), thromboxane B2(TxB2), PGF2αand PGD2by approximately 60 % in rats fed 10 % fish oil, and TxB2( − 50 %) and PGF2α( − 41 %) in rats fed 5 % fish oil. In conclusion, dietary fish oil prevented glomerular damage in MetS rats and mitigated the elevation in renal HETE levels. These results suggest a potential role for dietary fish oil to improve dysfunctional renal eicosanoid metabolism associated with kidney damage during conditions of the MetS. |
| Databáze: | OpenAIRE |
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