The TGF‐β family member derrière is involved in regulation of the establishment of left–right asymmetry
| Autor: | Norihisa Masuyama, Hiroshi Hanafusa, Hiroshi Shibuya, Eisuke Nishida, Morioh Kusakabe |
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| Rok vydání: | 2000 |
| Předmět: |
Embryo
Nonmammalian Microinjections Xenopus media_common.quotation_subject Blotting Western Molecular Sequence Data Embryonic Development Xenopus Proteins Biology Biochemistry Asymmetry Transforming Growth Factor beta Genetics Animals RNA Messenger Protein Precursors Growth Substances Molecular Biology In Situ Hybridization Body Patterning Glycoproteins media_common Scientific Reports Intracellular Signaling Peptides and Proteins Heart Zebrafish Proteins Molecular pathway biology.organism_classification Immunohistochemistry Cell biology Viscera Family member Oocytes Intercellular Signaling Peptides and Proteins Female Ectopic expression NODAL Transforming growth factor |
| Zdroj: | EMBO reports. 1:32-39 |
| ISSN: | 1469-3178 1469-221X |
| DOI: | 10.1093/embo-reports/kvd008 |
| Popis: | Although a number of genes that are involved in the establishment of left-right asymmetry have been identified, earlier events in the molecular pathway developing left-right asymmetry remain to be elucidated. Here we present evidence suggesting that the transforming growth factor-beta family member derrière is involved in the development of left-right asymmetry in Xenopus embryos. Ectopic expression of derrière on the right side can fully invert cardiac and visceral left-right orientation and nodal expression, and expression of a dominant-negative form of derrière on the left side can partially randomize the left-right orientation and nodal expression. Moreover, while expression of the dominant-negative derrière does not inhibit the activity of Vg1 directly, it can rescue the altered left-right orientation induced by Vg1. Vg1 can induce derrière in animal cap explants. These results suggest that derrière is involved in earlier molecular pathways developing the left-right asymmetry. |
| Databáze: | OpenAIRE |
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