Advances and applications of in-tube solid-phase microextraction for analysis of proteins
| Autor: | Xian-Hua Wang, Xuan Wang, Lin-Yi Dong, Rui Duan, Liang Xu, Yuan-Shuo Yang, Zhan-Song Hu |
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| Rok vydání: | 2021 |
| Předmět: |
Chromatography
Polymers Chemistry Aptamer 010401 analytical chemistry Organic Chemistry Molecularly imprinted polymer Proteins Restricted access Nanotechnology General Medicine Boronate affinity 010402 general chemistry Solid-phase microextraction Boronic Acids 01 natural sciences Biochemistry Antibodies 0104 chemical sciences Analytical Chemistry Molecular Imprinting Capillary column Solid Phase Microextraction |
| Zdroj: | Journal of Chromatography A. 1640:461962 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2021.461962 |
| Popis: | In-tube solid-phase microextraction (IT-SPME) with capillary column as extraction device is a well-established green extraction technique with a lot of applications in the fields of biomedicine, food and environment. This article reviews the research contributions of IT-SPME for analysis of proteins. The paper first briefly describes the history of IT-SPME. Then, the development and principle of IT-SPME for analysis of proteins are introduced, in which capillary column configurations of IT-SPME and instruments for quantitative analysis of proteins are summarized. Subsequently, the synthesis strategy and recognition principle of different recognition units, including antibodies, aptamers, molecularly imprinted polymers, and boronate affinity materials, are discussed in detail. This part also introduces several rare recognition units, including lectins, restricted access materials, lysine modified with β-cyclodextrin and cell membrane. The development trend and possible future direction of IT-SPME for analysis of proteins are mentioned. |
| Databáze: | OpenAIRE |
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