Effects of alkaline protease or restrictocin deficient mutants of Aspergillus fumigatus on human polymorphonuclear leukocytes
| Autor: | Y Ikegami, F Kuze, R Nawada, R Amitani, R Kawanami, Woon Joo Lee, Takako Murayama |
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| Rok vydání: | 1998 |
| Předmět: |
Pulmonary and Respiratory Medicine
Proteases Mutant Virulence Granulocyte In Vitro Techniques Microbiology Aspergillus fumigatus Fungal Proteins Ribonucleases Species Specificity Superoxides medicine Humans Protein Synthesis Inhibitors Aspergillus biology Chemotaxis Aspergillosis Allergic Bronchopulmonary Serine Endopeptidases Allergens Antigens Plant biology.organism_classification In vitro medicine.anatomical_structure Leukocytes Mononuclear |
| Zdroj: | The European respiratory journal. 12(3) |
| ISSN: | 0903-1936 |
| Popis: | Several substances including proteases and restrictocin have been suggested as candidates for virulence determinants in invasive pulmonary aspergillosis. However, the roles of such substances are not well understood. This study compared the in vitro suppressive effects of Aspergillus fumigatus culture filtrates (ACFs), on the functions of human polymorphonuclear leukocytes (PMNLs), the principal cells in the host defence against aspergillus hyphae, from a clinically isolated wild-type and isogenic mutant strains which lack production of elastolytic alkaline protease (Alp) and/or restrictocin. ACFs were obtained by culturing conidia of each strain in Medium- 199 at 37 degrees C for 5 days. ACFs of the wild-type significantly (p |
| Databáze: | OpenAIRE |
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