Down-regulation of cellular platelet-derived growth factor receptors induced by an activated neu receptor tyrosine kinase
| Autor: | Erkki Hölttä, B Westermark, L Lehtola, M Nistér, Kari Alitalo |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Platelet-derived growth factor
Receptor ErbB-2 medicine.medical_treatment Down-Regulation Receptors Cell Surface Gene Expression Regulation Enzymologic Receptor tyrosine kinase Mice 03 medical and health sciences chemistry.chemical_compound Transformation Genetic 0302 clinical medicine Epidermal growth factor Proto-Oncogene Proteins medicine Animals Receptors Platelet-Derived Growth Factor Growth factor receptor inhibitor Receptor 030304 developmental biology 0303 health sciences biology Growth factor 3T3 Cells Oncogenes General Medicine Protein-Tyrosine Kinases Molecular biology Gene Expression Regulation Neoplastic chemistry 030220 oncology & carcinogenesis biology.protein Tyrosine kinase Platelet-derived growth factor receptor Research Article |
| Zdroj: | Cell Regulation. 2:651-661 |
| ISSN: | 1044-2030 |
| Popis: | The functional integration of growth factor signaling occurs at several levels in target cells. One of the most proximal mechanisms is receptor transmodulation, by which one activated receptor can regulate the expression of other receptors in the same cells. Well-established transregulatory loops involve platelet-derived growth factor (PDGF) down-regulation of epidermal growth factor (EGF) receptors and beta-type transforming growth factors modulation of PDGF receptors. We have studied the relationship between neu tyrosine kinase activation and the expression of the PDGF receptors in transfected NIH/3T3 cells. Expression of the neu oncogene, but not of the neu proto-oncogene, was associated with a decrease of PDGF alpha- and beta-receptors on the cell surface, as measured by [125-I]PDGF-AA and -BB binding. These results were corroborated by metabolic labeling and immunoprecipitation of the PDGF beta-receptors. PDGF alpha- and beta-receptor mRNAs were strongly decreased in the neu oncogene-transformed cells in comparison with control cells expressing the neu proto-oncogene. Down-regulation of the PDGF receptors and their mRNAs was also observed after EGF treatment of cells expressing a chimeric EGF receptor/neu receptor, where the neu tyrosine kinase is activated by EGF binding. These results show that the neu tyrosine kinase can down-modulate PDGF receptor expression, and the effect is mediated via decreased PDGF receptor mRNA levels. |
| Databáze: | OpenAIRE |
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