Combination with Chemometrics and Quantification for Quality Evaluation and Variety Identification of Flos Chimonanthi Praecocis by HPLC
| Autor: | Chao Zhang, Lei Sun, Xing Yiwen, Jing-hua Su, Bing-ren Gu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Multifactor Dimensionality Reduction
Calycanthaceae Rutin Flos 01 natural sciences High-performance liquid chromatography Analytical Chemistry Chemometrics 0404 agricultural biotechnology Similarity (network science) Cluster Analysis Humans Immunologic Factors Medicine Chinese Traditional Chromatography High Pressure Liquid Principal Component Analysis Chromatography biology Chemistry Dimensionality reduction 010401 analytical chemistry Anti-Inflammatory Agents Non-Steroidal 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science 0104 chemical sciences Hierarchical clustering Principal component analysis Quercetin Quantitative analysis (chemistry) Drugs Chinese Herbal |
| Zdroj: | Journal of chromatographic science. 54(7) |
| ISSN: | 1945-239X |
| Popis: | Flos Chimonanthi Praecocis (FCP) is one of the popular traditional Chinese medicines, which have been widely used in China. Inconspicuous appearance differences are disadvantageous for identification, and it is difficult to evaluate the quality of FCP using the current methods. In this article, a simple method that combined chemometrics and quantitative analysis was established. The samples were obtained from three typical varieties for fingerprint analysis by high-performance liquid chromatography. Contents of rutin and quercetin were determined, and then a common pattern with 16 characteristic peaks was applied for principal component analysis, similarity analysis, and the hierarchical cluster analysis heatmap (HCA heatmap) to characterize the similarity and differences among samples for identification. Furthermore, seven characteristics peaks with higher loading values were selected for chemometrics analysis after dimensionality reduction to reduce analytical difficulty, and the new common pattern showed the similar identification effects. Overall, combination with chemometrics and quantitative analysis would provide a useful and simple method for quality control of FCP in the future. |
| Databáze: | OpenAIRE |
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