Secondary metabolites of Aspergillus exert immunobiological effects on human monocytes
| Autor: | V. Cusumano, Elisabetta Buommino, E. Losi, Maria Rosaria Catania, Fabio Rossano, Loredana Ortega De Luna |
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| Přispěvatelé: | Rossano, Fabio, Ortega De Luna, L, Buommino, Elisabetta, Cusumano, V, Losi, E, Catania, MARIA ROSARIA, F., Rossano, L., ORTEGA DE LUNA, V., Cusumano, E., Losi, RESEARCH IN MICROBIOLOGY, M. R. C. A. T. A. N. I. A. |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Aflatoxin B1
Time Factors Lipopolysaccharide medicine.medical_treatment Biology medicine.disease_cause Microbiology chemistry.chemical_compound Gene expression medicine Protein biosynthesis Humans Molecular Biology Cytokine Messenger RNA Dose-Response Relationship Drug Toxin Monocyte General Medicine Aspergillu Molecular biology Aspergillus medicine.anatomical_structure chemistry Carcinogens Leukocytes Mononuclear Cytokines Tumor necrosis factor alpha Carcinogen Human |
| Popis: | This project focused on the effects of aflatoxin B1 (AFB1), a food-contaminating mycotoxin produced by fungi, genus Aspergillus, on the release and genetic expression of some important cytokines, i.e., (interleukin-1 alpha (IL-1 alpha), IL-6, tumor necrosis factor-alpha (TNF alpha)) by human monocytes. Monocytes, preincubated for different time periods with concentrations of AFB1 ranging from 0.01 to 1.0 pg/mL, were then activated with bacterial lipopolysaccharide. Cytokine levels were measured by immunoassay and mRNA by cDNA amplification. Pretreatment of monocytes with AFB1 resulted in a decrease in IL-1, IL-6 and TNF alpha release already at a concentration of 0.05 pg/mL. The gene expression of the cytokines considered was drastically affected by treatment with AFB1. In fact, AFB1 completely blocked the transcription of IL-1 alpha, IL-6 and TNF alpha mRNAs, while it did not affect beta-actin mRNA at the concentrations used. It therefore appears that AFB1 exerts its effect on cytokine release through selective inhibition of specific mRNA, without affecting general protein synthesis. |
| Databáze: | OpenAIRE |
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