Fire blight in Georgia

Autor: Dali Gaganidze, Mariam Aznarashvili, Ekaterine O. Abashidze, Tinatin Sadunishvili, Manana A. Gureilidze, Eter S. Gvritishvili
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Annals of Agrarian Science, Vol 16, Iss 1, Pp 12-16 (2018)
ISSN: 1512-1887
Popis: Fire blight is distinguished among the fruit tree diseases by harmfulness. Fire blight damages about 180 cultural and wild plants belonging to the Rosaceae family. Quince, apple and pear are the most susceptible to the disease. At present, the disease occurs in over 40 countries of Europe and Asia. Economic damage caused by fire blight is expressed not only in crop losses, but also, it poses threat of eradication to entire fruit tree gardens. Erwinia amylovora, causative bacteria of fire blight in fruit trees, is included in the A2 list of quarantine organisms. In 2016, the employees of the Plant Pest Diagnostic Department of the Laboratory of the Georgian Ministry of Agriculture have detected Erwinia amylovora in apple seedlings from Mtskheta district. National Food Agency, Ministry of Agriculture of Georgia informed FAO on pathogen detection. The aim of the study is detection of the bacterium Erwinia amylovora by molecular method (PCR) in the samples of fruit trees, suspicious on fire blight collected in the regions of Eastern (Kvemo Kartli, Shida Kartli and Kakheti) and Western Georgia (Imereti).The bacterium Erwinia amylovora was detected by real time and conventional PCR methods using specific primers and thus the fire blight disease confirmed in 23 samples of plant material from Shida Kartli (11 apples, 6 pear and 6 quince samples), in 5 samples from Kvemo Kartli (1 quince and 4 apple samples), in 2 samples of apples from Kakheti region and 1 sample of pear collected in Imereti (Zestafoni). Keywords: Fire blight, Erwinia amylovora, Conventional PCR, Real time PCR, DNA, Bacterium
Databáze: OpenAIRE