Antigenic competition in a multivalent foot rot vaccine
| Autor: | Jacqueline D. Hunt, David J. Stewart, Paul R. Wood, Lorena E. Brown, David C. Jackson |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Recombinant Fusion Proteins Fimbria Immunization Secondary Sheep Diseases Dichelobacter nodosus Biology Pilus Microbiology Bacteria Anaerobic Antigen Foot rot Animals Bacteroides Foot Rot Antigens Bacterial Sheep General Veterinary General Immunology and Microbiology Polyvalent Vaccine Vaccination Public Health Environmental and Occupational Health biology.organism_classification Virology Antibodies Bacterial Infectious Diseases Agglutinins Fimbriae Bacterial Pseudomonas aeruginosa biology.protein Molecular Medicine Fimbriae Proteins Antibody Bacterial Outer Membrane Proteins |
| Zdroj: | Vaccine. 12(5) |
| ISSN: | 0264-410X |
| Popis: | The antigenic competition that occurs when pilus antigens of different serogroups are combined in multivalent vaccines for foot rot has been investigated using recombinant pilus antigens. Our prototype vaccine contains pili from nine serogroups of Dichelobacter nodosus which are expressed in Pseudomonas aeruginosa. Sheep inoculated with this multivalent vaccine were not as well protected against foot rot as those given the monovalent vaccine. Levels of agglutinating and total antibody specific for any particular pili serogroup were found to be significantly reduced in sheep vaccinated with six or more closely related pili. This effect was more pronounced for agglutinating antibody, which is thought to mediate protection, but was also observed with total antibody levels measured by ELISA. The antigenic competition was not associated with the total antigen load as a tenfold higher dose of monovalent pili induced high titres of antibody. Furthermore, distributing the vaccine to four sites, each draining to a different lymph node, failed to overcome the competition. Experiments with mixtures of monospecific sera indicate that the phenomenon is unlikely to be due to blocking of serogroup-specific protective antibodies by an excess of cross-reactive non-protective antibody elicited by heterologous pili. |
| Databáze: | OpenAIRE |
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