Efficient conversion to Cypridina luciferin from Cypridina luciferyl sulfate, coupled with enzymatic sulfation of acetic acid
| Autor: | Satoshi Inouye, Kohsuke Ishizuka, Kazuo Matsuda, Mitsuhiro Nakamura, Takashi Morikawa |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Sulfotransferase Luminescence Biophysics Biochemistry Medicinal chemistry Catalysis 03 medical and health sciences Acetic acid chemistry.chemical_compound 0302 clinical medicine Sulfation Crustacea Animals Luciferase Sulfate Luciferases Molecular Biology Phosphoric acid Acetic Acid Luminescent Agents Sulfates Chemistry Imidazoles Cell Biology Luciferin 030104 developmental biology Pyrazines 030220 oncology & carcinogenesis Luminescent Measurements Sulfotransferases |
| Zdroj: | Biochemical and Biophysical Research Communications. 529:678-684 |
| ISSN: | 0006-291X |
| Popis: | In Cypridina (Vargula) hilgendorfii, Cypridina luciferin is converted from Cypridina luciferyl sulfate by a sulfotransferase with adenosine 3′, 5′-diphosphate (PAP), and is used for the luminescence reaction of Cypridina luciferase. We found that the luminescence activity of crude extracts of C. hilgendorfii was significantly stimulated by the addition of acetic acid. This stimulation may be explained by an efficient supply of PAP from 3′-phosphoadenosine 5′-phosphosulfate (PAPS) catalyzed by a sulfotransferase. Thus, acetic acid acts as a sulfate acceptor from PAPS, followed by forming acetyl sulfate and PAP. The structure of acetyl sulfate was identified using mass spectrometry and it spontaneously decomposed to acetic acid and free sulfate ion in aqueous solutions. This enzymatic conversion from Cypridina luciferyl sulfate to Cypridina luciferin could be coupled with acetic acid and PAPS by a sulfotransferase. |
| Databáze: | OpenAIRE |
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