Anti-MUC1 Aptamer as Carrier Tool of the Potential Radiosensitizer 1,10 Phenanthroline in MCF-7 Breast Cancer Cells
| Autor: | Sotiris Missailidis, Laís Nascimento Alves, Carlos Eduardo Bonacossa de Almeida, Claudia Lage |
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| Rok vydání: | 2019 |
| Předmět: |
Radiation-Sensitizing Agents
Cancer Research Radiosensitizer Aptamer Breast Neoplasms Flow cytometry 03 medical and health sciences 0302 clinical medicine medicine Humans Cytotoxic T cell Ferrous Compounds MUC1 Drug Carriers medicine.diagnostic_test Chemistry Mucin-1 General Medicine Aptamers Nucleotide Endocytosis Oncology MCF-7 030220 oncology & carcinogenesis Drug delivery Cancer cell MCF-7 Cells Cancer research Feasibility Studies Female Phenanthrolines |
| Zdroj: | Anticancer Research. 39:1859-1867 |
| ISSN: | 1791-7530 0250-7005 |
| Popis: | Background Proteins overexpressed in malignant tissues form important targets in the development of targeted therapeutics, and aptamers comprise an important affinity agent for therapy and drug delivery. In this study, aberrantly expressed mucin 1 glycoprotein was investigated as a therapeutic target in a breast cancer model. Materials and methods In order to determine the feasibility of using an aptamer against mucin 1 (aptA) as carrier of the cytotoxic compound 1,10-phenanthroline to MCF-7 cells, as a potential radiosensitizer, was studied in experiments using circular dichroism and rhodamine labelling by fluorescent microscopy and flow cytometry. Results 1,10-Phenanthroline can be intercalated within aptA when complexed with Fe(II) ions, with dissociation constant (Kd) of 30 μM. The complex was subsequently capable of binding to and being internalised in MCF-7 breast cancer cells. Conclusion aptA can carry 1,10-phenanthroline to cancer cells specifically and this complex represents a potential target-directed anticancer therapy. |
| Databáze: | OpenAIRE |
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