Transcriptional Hallmarks of Heterogeneous Neural Stem Cell Niches of the Subventricular Zone
| Autor: | Stefan Zweifel, Kasum Azim, Bruno Fischer, Verdon Taylor, Olivier Raineteau, Anahi Hurtado-Chong, Nitin Kumar |
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| Předmět: |
animal diseases
Subventricular zone Gene Expression Biology Genome Transcriptome Lateral ventricles Mice Neural Stem Cells Lateral Ventricles medicine Animals Stem Cell Niche Transcription factor Gene Cell Biology Anatomy Neural stem cell Cell biology Mice Inbred C57BL medicine.anatomical_structure nervous system Molecular Medicine Stem cell Developmental Biology Transcription Factors |
| Zdroj: | Stem cells (Dayton Ohio) STEM CELLS |
| DOI: | 10.1002/stem.2017 |
| Popis: | Throughout postnatal life in mammals, neural stem cells (NSCs) are located in the subventricular zone (SVZ) of the lateral ventricles. The greatest diversity of neuronal and glial lineages they generate occurs during early postnatal life in a region-specific manner. In order to probe heterogeneity of the postnatal SVZ, we microdissected its dorsal and lateral walls at different postnatal ages and isolated NSCs and their immediate progeny based on their expression of Hes5-EGFP/Prominin1 and Ascl1-EGFP, respectively. Whole genome comparative transcriptome analysis revealed transcriptional regulators as major hallmarks that sustain postnatal SVZ regionalization. Manipulation of single genes encoding for locally enriched transcription factors (loss-of-function or ectopic gain-of-function in vivo) influenced NSC specification indicating that the fate of regionalized postnatal SVZ-NSCs can be readily modified. These findings reveal the pronounced transcriptional heterogeneity of the postnatal SVZ and provide targets to recruit region-specific lineages in regenerative contexts. Stem Cells 2015;33:2232–2242 |
| Databáze: | OpenAIRE |
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