The interleukin-1-stimulated protein kinase that phosphorylates heat shock protein hsp27 is activated by MAP kinase
| Autor: | P. Delaney, G.D. Virca, Steven K. Dower, P R Sleath, H.D. Schule, Timothy A. Bird, P. de Roos |
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| Rok vydání: | 1994 |
| Předmět: |
endocrine system
animal structures Molecular Sequence Data Biophysics Protein Serine-Threonine Kinases Signal transduction Mitogen-activated protein kinase kinase Peptide Mapping Biochemistry Protein kinase MAP2K7 Protein phosphorylation Structural Biology Tumor Cells Cultured Genetics Humans Amino Acid Sequence c-Raf Phosphorylation Molecular Biology Heat-Shock Proteins MAPK14 Mitogen-Activated Protein Kinase 1 MAP kinase kinase kinase biology MAPKAPK2 Cyclin-dependent kinase 2 Intracellular Signaling Peptides and Proteins Cell Biology Protein-Tyrosine Kinases Molecular biology Recombinant Proteins Enzyme Activation Kinetics biology.protein Cyclin-dependent kinase 9 Interleukin-1 |
| Zdroj: | FEBS Letters. 338:31-36 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(94)80111-8 |
| Popis: | In KB cells, interleukin-1 (IL-1), epidermal growth factor and phorbol ester transiently activated both MAP kinase and a serine kinase which phosphorylated the heat shock protein hsp27. Extracts made from IL-1-stimulated KB cells phosphorylated recombinant hsp27, in vitro, on serine residues 78 and 82 which are contained within Arg-X-X-Ser motifs similar to those phosphorylated by the ribosomal protein S6 kinases. Upon size exclusion chromatography, however, hsp27 kinase eluted as a single peak of activity at 50–60 kDa, clearly separated from ribosomal protein S6 kinases. Treatment of partially purified hsp27 kinase with protein phosphatase-2a reduced its activity by 80%. De-phosphorylated hsp27 kinase could be approximately 50% reactivated by a factor present in IL-1-treated cell extracts in the presence of ATP. This factor co-eluted with MAP kinase after partial purification by DEAE-cellulose, phenyl Sepharose, and size exclusion chromatography. Purified sea star p44mpk and recombinant ERK2 MAP kinases were also capable of re-activating hsp27 kinase to a similar extent. These data suggest that hsp27 kinase is downstream from, and probably a direct target of MAP kinase. |
| Databáze: | OpenAIRE |
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