Multiple Rounds of Artificial Selection Promote Microbe Secondary Domestication—The Case of Cachaça Yeasts
| Autor: | Gabriela Montandon, Ana Pontes, Renata O. Santos, Paula Gonçalves, Camila M de Ponzzes-Gomes, Paula B. Morais, Raquel Barbosa, José Paulo Sampaio, Carlos A. Rosa |
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| Přispěvatelé: | UCIBIO - Applied Molecular Biosciences Unit, DCV - Departamento de Ciências da Vida |
| Rok vydání: | 2018 |
| Předmět: |
domestication traits
0301 basic medicine cachaça fermentation Saccharomyces cerevisiae Proteins yeast evolutionary biology Genes Fungal Population Saccharomyces cerevisiae comparative and population genomics Biology Aquaporins Models Biological 03 medical and health sciences Genetics Selection Genetic education Domestication Phylogeny Ecology Evolution Behavior and Systematics Selection (genetic algorithm) microbe population genomics microbe domestication 2. Zero hunger Wine Comparative genomics Fermentation in winemaking education.field_of_study Base Sequence business.industry cachaca fermentation Genetic Variation food and beverages biology.organism_classification Biotechnology 030104 developmental biology Fermentation business Research Article |
| Zdroj: | Genome Biology and Evolution |
| ISSN: | 1759-6653 |
| DOI: | 10.1093/gbe/evy132 |
| Popis: | This work was supported by Fundac¸ão para a Ciência e a Tecnologia (Portugal) grants PTDC/AGR-ALI/118590/2010 and PTDC/BIA-EVF/118618/2010 (J.P.S., P.G.), and UID/ Multi/04378/2013 (A.P., J.P.S., P.G.). This work was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq—Brazil, process numbers 407415/2013 and 0457499/2014-1, C.A.R.), Fundac¸ão do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG, process number APQ-01525-14, C.A.R.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The study of microbe domestication has witnessed major advances that contribute to a better understanding of the emergence of artificially selected phenotypes and set the foundations of their rational improvement for biotechnology. Several features make Saccharomyces cerevisiae an ideal model for such a study, notably the availability of a catalogue of signatures of artificial selection and the extensive knowledge available on its biological processes. Here, we investigate with population and comparative genomics a set of strains used for cachaça fermentation, a Brazilian beverage based on the fermentation of sugar cane juice. We ask if the selective pressures posed by this fermentation have given rise to a domesticated lineage distinct from the ones already known, like wine, beer, bread, and sake yeasts. Our results show that cachaça yeasts derive from wine yeasts that have undergone an additional round of domestication, which we define as secondary domestication. As a consequence, cachaça strains combine features of wine yeasts, such as the presence of genes relevant for wine fermentation and advantageous gene inactivations, with features of beer yeasts like resistance to the effects of inhibitory compounds present in molasses. For other markers like those related to sulfite resistance and biotin metabolism our analyses revealed distributions more complex than previously reported that support the secondary domestication hypothesis. We propose a multilayered microbe domestication model encompassing not only transitions from wild to primarily domesticated populations, as in the case of wine yeasts, but also secondary domestications like those of cachaça yeasts. authorsversion published |
| Databáze: | OpenAIRE |
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