The maize fused leaves1 (fdl1) gene controls organ separation in the embryo and seedling shoot and promotes coleoptile opening

Autor: David S. Horner, Martina Persico, Marina Cavaiuolo, D. Gabotti, Gabriella Consonni, Peter M. Rogowsky, Francesca Dalla Vecchia, Alessandra Barbante, Jelena Krstajic, Ghislaine Gendrot, Nicoletta Rascio, Priscilla S. Manzotti, Alessio Scarafoni, Nicoletta La Rocca
Přispěvatelé: Dipartimento di Biologia, Universita degli Studi di Padova, Università degli Studi di Milano [Milano] (UNIMI), Reproduction et développement des plantes (RDP), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Dipartimento di Bioscienze, University of Parma = Università degli studi di Parma [Parme, Italie], Università degli Studi di Padova = University of Padua (Unipd), Università degli Studi di Milano = University of Milan (UNIMI), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Università degli studi di Parma = University of Parma (UNIPR)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Journal of Experimental Botany
Journal of Experimental Botany, Oxford University Press (OUP), 2015, pp.1-15. ⟨10.1093/jxb/erv278⟩
Journal of Experimental Botany, 2015, pp.1-15. ⟨10.1093/jxb/erv278⟩
ISSN: 0022-0957
1460-2431
DOI: 10.1093/jxb/erv278⟩
Popis: Highlight This study provides the first characterization of an R2R3 family MYB transcription factor involved in cuticle and epicuticular wax deposition, whose action is confined to maize embryogenesis and juvenile phase.
The fdl1-1 mutation, caused by an Enhancer/Suppressor mutator (En/Spm) element insertion located in the third exon of the gene, identifies a novel gene encoding ZmMYB94, a transcription factor of the R2R3-MYB subfamily. The fdl1 gene was isolated through co-segregation analysis, whereas proof of gene identity was obtained using an RNAi strategy that conferred less severe, but clearly recognizable specific mutant traits on seedlings. Fdl1 is involved in the regulation of cuticle deposition in young seedlings as well as in the establishment of a regular pattern of epicuticular wax deposition on the epidermis of young leaves. Lack of Fdl1 action also correlates with developmental defects, such as delayed germination and seedling growth, abnormal coleoptile opening and presence of curly leaves showing areas of fusion between the coleoptile and the first leaf or between the first and the second leaf. The expression profile of ZmMYB94 mRNA—determined by quantitative RT-PCR—overlaps the pattern of mutant phenotypic expression and is confined to a narrow developmental window. High expression was observed in the embryo, in the seedling coleoptile and in the first two leaves, whereas RNA level, as well as phenotypic defects, decreases at the third leaf stage. Interestingly several of the Arabidopsis MYB genes most closely related to ZmMYB94 are also involved in the activation of cuticular wax biosynthesis, suggesting deep conservation of regulatory processes related to cuticular wax deposition between monocots and dicots.
Databáze: OpenAIRE
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