Different antibody responses against hepatitis B surface antigen among mouse strains transfected with mutant viral DNA
| Autor: | Naoko Kanai, Miho Ogawa, Naoaki Hayashi, Riho Joh, Aiko Iizuka, Kiyoshi Hasegawa, Nobuyuki Torii, Takuma Naritomi |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Hepatitis B virus HBsAg Hepatitis B virus DNA polymerase viruses Molecular Sequence Data Viral transformation Biology medicine.disease_cause Sensitivity and Specificity Hepatitis B virus PRE beta Mice Antigen medicine Animals Humans Hepatitis B Antibodies Fulminant hepatitis Mice Inbred BALB C Hepatitis B Surface Antigens Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gastroenterology Hepatitis B medicine.disease Immunohistochemistry Virology Molecular biology Disease Models Animal Hepatic Encephalopathy Antibody Formation Mutation RNA Viral Female |
| Zdroj: | Journal of Gastroenterology. 37:1035-1041 |
| ISSN: | 1435-5922 0944-1174 |
| DOI: | 10.1007/s005350200174 |
| Popis: | Rapid seroconversion from hepatitis B surface antigen (HBsAg) to anti-HBs antibody is seen in most patients with fulminant hepatitis B. It is unclear whether viral mutation or host immune background is responsible for such enhanced host reaction to hepatitis B virus (HBV). To investigate interaction between virus mutation and host immune background, we established a mouse model of hepatitis B using liposome-mediated gene transfer. Methods: A mixture of liposomes and full-length viral DNA derived from hepatitis B patients was injected into three strains of purebred mice intrahepatically. After injection, HBsAg and antibody in liver and serum were serially measured. Results: Three days after transfection, viral transcript and antigen were detected in the liver and serum. Ten days after transfection with wild-type DNA, hepatitis B surface antibody was detectable in two of the three strains. Mice that did not produce antibody after transfection with wild-type DNA produced a high amount of serum antibody against surface antigen when transfected with fulminant hepatitis-associated DNA. Conclusions: The present results are consistent with previous clinical observations of rapid HBsAg seroconversion in patients with fulminant hepatitis B. Further studies are needed to determine which mutations are responsible for differences in immunogenicity between HBV strains. |
| Databáze: | OpenAIRE |
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