B beta(15-42) (FX06) reduces pulmonary, myocardial, liver, and small intestine damage in a pig model of hemorrhagic shock and reperfusion
| Autor: | Nguyen Tran, Peter Petzelbauer, Brigitte Vollmar, Thomas Iber, Dierk A. Vagts, Kai Zacharowski, Alexander Koch, G. Nöldge-Schomburg, Thomas Scheeren, Jan P. Roesner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
pig EXTRAVASCULAR LUNG WATER medicine.medical_specialty Pathology BLOOD Swine Cardiac index fibrin-derived peptide organ injury and reperfusion KAPPA-B Shock Hemorrhagic Lung injury Critical Care and Intensive Care Medicine Fibrin Fibrinogen Degradation Products hemorrhagic shock ISCHEMIA-REPERFUSION Intensive care Internal medicine Intestine Small INJURY Animals Humans Medicine Lung PEPTIDE B-BETA(15-42) NITRIC-OXIDE business.industry RESUSCITATION INFLAMMATORY RESPONSE Lung Injury Stroke volume medicine.disease Peptide Fragments Small intestine BINDING-PROTEIN Endocrinology medicine.anatomical_structure Heart Injuries Liver Reperfusion Injury Shock (circulatory) Models Animal medicine.symptom business Reperfusion injury |
| Zdroj: | Critical Care Medicine, 37(2), 598-605. LIPPINCOTT WILLIAMS & WILKINS |
| ISSN: | 0090-3493 |
| Popis: | The fibrin-derived peptide Bbeta15-42 (also called FX06) has been shown to reduce myocardial infarct size following ischemia/reperfusion. Hemorrhagic shock (HS) followed by volume resuscitation represents a similar scenario, whereby a whole organism is vulnerable to reperfusion injury.We subjected male farm-bred landrace pigs ( approximately 30 kg) to HS by withdrawing blood to a mean arterial pressure of 40 mm Hg for 60 minutes. Pigs were then resuscitated with shed blood and crystalloids for 60 minutes, and at this time, FX06 (2.4 mg/kg, n = 8) or vehicle control (phosphate buffered saline; 2.4 mg/kg, n = 7) was injected as an intravenous bolus.University hospital laboratory.Anesthetized male farm-bred landrace pigs.Data are presented as mean +/- sd. Five hours after resuscitation, controls presented acute lung injury (Pao2/Fio2-ratio300 mm Hg; extra-vascular lung water index (marker for lung injury): 9.0 +/- 1.8 mL/kg) and myocardial dysfunction/damage (cardiac index: 4.3 +/- 0.25 L/min/m; stroke volume index: 30 +/- 6 mL/m; cardiac TnT levels: 0.58 +/- 0.25 ng/mL). In contrast, FX06-treated animals showed significantly improved pulmonary and circulatory function (Pao2/Fio2-ratio*400 mm Hg; extra-vascular lung water index: *5.2 +/- 2.1 mL/kg, cardiac index: *6.3 +/- 1.4 L/min/m; stroke volume index: *51 +/- 11 mL/m; cardiac TnT levels: *0.11 +/- 0.09 ng/mL; *p0.05). Also, tissue oxygenation (tpO2; mm Hg) was significantly improved during reperfusion in FX06-treated pigs when compared with controls (liver 51 +/- 4 vs. *65 +/- 4; serosa 44 +/- 5 vs. *55 +/- 7; mucosa 14 +/- 4 vs. *26 +/- 4). Finally, FX06 reduced accumulation of myeloperoxidase-positive cells (mainly neutrophils) in myocardium, liver, and small intestine and reduced interleukin-6 plasma levels (*p0.05; compared with controls).We conclude that in a pig model of HS and reperfusion, administration of FX06 during reperfusion protects shock- susceptible organs such as heart, lung, liver, and small intestine. |
| Databáze: | OpenAIRE |
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