Regulation of myosin IIA and filamentous actin during insulin-stimulated glucose uptake in 3T3-L1 adipocytes
| Autor: | Joseph Ramos, Richard Stall, Yashomati M. Patel, F. Kent Fulcher |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Glucose uptake
macromolecular substances Biology Filamentous actin Article Cell membrane Mice Calmodulin 3T3-L1 Cells Myosin Adipocytes medicine Animals Insulin Glucose Transporter Type 4 Nonmuscle Myosin Type IIA Cell Membrane Glucose transporter Colocalization Cell Biology Actin cytoskeleton Actins Cell biology Actin Cytoskeleton Glucose medicine.anatomical_structure biology.protein GLUT4 |
| Zdroj: | Experimental Cell Research. 322:81-88 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/j.yexcr.2013.12.011 |
| Popis: | Insulin stimulated glucose uptake requires the colocalization of myosin IIA (MyoIIA) and the insulin-responsive glucose transporter 4 (GLUT4) at the plasma membrane for proper GLUT4 fusion. MyoIIA facilitates filamentous actin (F-actin) reorganization in various cell types. In adipocytes F-actin reorganization is required for insulin-stimulated glucose uptake. What is not known is whether MyoIIA interacts with F-actin to regulate insulin-induced GLUT4 fusion at the plasma membrane. To elucidate the relationship between MyoIIA and F-actin, we examined the colocalization of MyoIIA and F-actin at the plasma membrane upon insulin stimulation as well as the regulation of this interaction. Our findings demonstrated that MyoIIA and F-actin colocalized at the site of GLUT4 fusion with the plasma membrane upon insulin stimulation. Furthermore, inhibition of MyoII with blebbistatin impaired F-actin localization at the plasma membrane. Next we examined the regulatory role of calcium in MyoIIA-F-actin colocalization. Reduced calcium or calmodulin levels decreased colocalization of MyoIIA and F-actin at the plasma membrane. While calcium alone can translocate MyoIIA it did not stimulate F-actin accumulation at the plasma membrane. Taken together, we established that while MyoIIA activity is required for F-actin localization at the plasma membrane, it alone is insufficient to localize F-actin to the plasma membrane. |
| Databáze: | OpenAIRE |
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