Randomized comparison of two commercial culture media (Cook and Vitrolife) for embryo culture after IMSI
| Autor: | Bruna Petersen, Vanessa A. Comar, Joao Batista A. Oliveira, Claudia G. Petersen, Felipe Dieamant, Adriana Renzi, Juliana Ricci, Ana L. Mauri, Ricardo L. R. Baruffi, Jose G. Franco, Laura D. Vagnini, Mariana C Matilla |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine culture medium MSOME Biology Embryo Culture Techniques Andrology Random Allocation 03 medical and health sciences 0302 clinical medicine Human fertilization Pregnancy medicine Humans Embryo Implantation Prospective Studies Sperm Injections Intracytoplasmic Blastocyst 030219 obstetrics & reproductive medicine Incubator Embryo culture Embryo Commercial culture Embryo Transfer Embryo Mammalian medicine.disease human embryo Culture Media 030104 developmental biology medicine.anatomical_structure Original Article Female Embryo quality |
| Zdroj: | JBRA Assisted Reproduction |
| ISSN: | 1518-0557 |
| DOI: | 10.5935/1518-0557.20180058 |
| Popis: | Objective A variety of studies randomizing women/cycles or oocytes/embryos has been carried out to compare different culture media for culturing embryos up to cleavage or blastocyst stages showing controversial results. A recent systematic review suggested that data in the literature are insufficient to conclude the best culture medium for embryo quality, pregnancy and implantation. The objective of this study was to evaluate whether there is any difference between two commercial culture media regarding clinical outcomes after IMSI cycles. Methods A total of 120 patients, ≤39 years of age, undergoing ART treatment submitted to the IMSI program were prospectively broken down and randomized into two groups: Group I (Cook media) and Group II (Vitrolife media). Results Our data demonstrated that there was no difference using all the media from Cook or all the media from Vitrolife, for culturing embryos till day 2, in the bench incubator at low O2 concentration, in relation to fertilization, embryo quality, pregnancy and implantation rates (p>0.05). Conclusion Both culture media used, Cook medium and Vitrolife medium, for the IMSI procedure and for later embryo culture with transfer on the second day, are equally effective and can be used depending on the ease and availability of acquisition. |
| Databáze: | OpenAIRE |
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