Artificial turn-on riboswitch to control target gene expression using a wild-type riboswitch splicing mechanism
| Autor: | Shuji Hirohata, Takahiro Yamauchi, Daisuke Miyoshi, Naoki Sugimoto, Takafumi Kubodera |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Riboswitch Aspergillus oryzae RNA Splicing Gene Expression Bioengineering Computational biology 01 natural sciences Applied Microbiology and Biotechnology Neurospora crassa 03 medical and health sciences 010608 biotechnology Gene expression Gene Regulation of gene expression biology Chemistry Alternative splicing Wild type food and beverages biology.organism_classification 030104 developmental biology RNA splicing Thiamine Pyrophosphate Genetic Engineering Biotechnology |
| Zdroj: | Journal of bioscience and bioengineering. 131(2) |
| ISSN: | 1347-4421 |
| Popis: | The thiamine pyrophospate (TPP)-dependent thiA riboswitch in Aspergillus oryzae regulates alternative mRNA splicing via TPP-riboswitch binding to reduce protein production. Based on the sequences involved in alternative splicing found in Neurospora crassa, we identified unique sequences that are thought to play a role in the alternative splicing of the thiA riboswitch. Based on the mechanism of alternative splicing regulated by the thiA riboswitch, we constructed a new TPP-dependent artificial riboswitch, which unlike the wild-type riboswitch, promotes, rather than inhibits, gene expression. We found that a target gene controlled by this turn-on artificial riboswitch can be sufficiently expressed for practical use in A. oryzae. The artificial riboswitch upregulates the target genes via TPP and can be applied as a practical tool for gene regulation. |
| Databáze: | OpenAIRE |
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