FIGURE 1 from Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans

Autor:	Seung-Oe Lim, Deborah W. Knapp, J. Paul Robinson, Valery Patsekin, Robert H. Carnahan, Uma K. Aryal, Jackeline Franco, Raluca Ostafe, Perry M. Kirkham, Deepika Dhawan, Alyssa Min Jung Kim, Wonkyung Oh
Rok vydání:	2023
DOI:	10.1158/2767-9764.22822025
Popis:	Working flow chart for production and validation of the cPD-L1 antibody. A, Immunization of antigen, cPD-L1 protein. B, Establishment of hybridomas (over 2,000 clones). C, Evaluation of the cPD-L1 specific antibody by the live cell–based antibody binding assay. D, Therapeutic antibody selection by cPD-L1/cPD-1 blockade assay. E,In vivo validation of therapeutic efficacy of cPD-L1 antibodies in the caninized PD-L1 mice. F, Production of the cPD-L1 chimeric antibody. G, Validation of the cPD-L1 chimeric antibody. H, Initial safety profile and PK analysis. PK, pharmacokinetic.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f349bf5c084626a7d27c1d75041bb85 https://doi.org/10.1158/2767-9764.22822025 Zobrazit plný text záznamu